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Purification and characterization of a lectin from Jingshan 471 variety of sweetpotato

LIU Yan ru 1, YU Ping 2, CHEN Feng xiang 3(1.Biochemistry Lab, Institute of Polymer Science; 2.Institute of Polymer Science, Fujian Teachers' University, Fuzhou, Fujian 350007, China; 3.College of Crop Science, Fujian Agricultural University, Fuzhou,  
Sweetpotato Lectin(SPL) was isolated from lamima textus of Jinshan 471, a variety of Ipomoea batatas Poir, by ammonium sulfate fractional precipitation, chitin affinity chromatography column and sephadex G 75. By PAGE or SDS PAGE, it presented to be a protein belt. Subunit relative molecular mass measured by SDS PAGE is about 41×10 3. SPL can agglutinate various human blood types and some zoic akaryovyte, among these, the agglutination activity of SPL in pigeon akaryocyte is the highest. SPL can agglutinate S 180 sarcoma cell of mice. The agglutinative activity of SPL can be suppressed by arabinose, GluNAe, etc. After heating for 20 minutes at 90 ℃, SPL can be entirely devitalized. The neutro saccharide content of SPL is 8.42%. The amido acid composition analysis suggested that SPL molecules abundantly contained acid amido acid.
【Fund】: 福建省自然科学基金资助项目!(C9810 0 12 )
【CateGory Index】: Q946.1
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