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《Journal of Fujian Agricultural University(Natural Science)》 2001-03
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Studies on DNA extraction and establishment of RAPD reaction system in jute

ZHOU Dong xin, QI Jian min,WU Wei ren, LI Wei ming (College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China)  
Method of DNA extraction and factors influencing RAPD analysis,including the concentration of DNA template, Mg 2+ , dNTP, primers and Taq, in jute were studied. An optimal PCR system for RAPD analysis in jute was found: in 25 μL reaction solution, contained 10 mmol·L -1 Tris HCl(pH8.0),50 mmol·L -1 KCl,2.5 mmol·L -1 MgCl 2,150 μmol·L -1 dNTP, 0.2 μmol·L -1 random primer, 30-60 ng DNA template, 1.5 U Taq polymerase. The amplification program was devised:94 ℃ for 5 min; denaturing at 94 ℃ for 30 s, primer annealing at 37 ℃ for 1.5 min, extension at 72 ℃ for 1 min, 41 cycles; at last extension 72 ℃ for 7 min.
【CateGory Index】: S563.4
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