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《Journal of Fujian Agricultural University(Natural Science)》 2002-03
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A review on some technical problems in RAPD application

JIANG Zi feng, LIN Nai quan, XU Mei(Biological Control Research Institute, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China)  
Random amplified polymorphic DNA (RAPD) technique as a fast, economic, less sample reqired and more polymorphic method has been successfully used in detecting genetic polymorphism, location of different genes, identification of races, medical diagnosis, construction of genetic maps, systematic research etc. But its reliability has been suspected owing to poor repeatability and homology of co migrating bands in the experiment. A general review on some aspects, such as the ways of sampling and conservation, repeatability, homology of co migrating bands, pollution, standardization in publication etc., is given in this paper and some suggestions are also provided accordingly.
【CateGory Index】: Q781
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