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《Journal of Fujian Agricultural University(Natural Science)》 2002-04
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Applicability of inter-simple sequence repeat polymorphisms in sugarcane and its related genera as DNA markers

YU Aili, ZHANG Muqing, CHEN Rukai Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China)  
One hunderd anchored SSR primers from UBC#9 were used for PCR amplification from a sample set of 11 genotypes including individuals of S.spontaneum, S.sinense, S.barberi, S.robustom, S.officinarum, and other wild relatives, Erianthus rockii, E. arundinaceus and Miscanthus floridulus. Amplification products were resolved in 1.5% agarose gels and banding patterns were compared among the 11 genotypes. Of the 100 primers tested, 32 produced clear banding patterns that could be easily scored. Nineteen of these 32 primers were designed to dinucleotide repeats, three to trinucleotide repeats, two to tetranucleotide repeats, three to pentannucleotide repeats. Four primers designed to 5′ degenerate motifs also yielded discrete repeats. All primers designed to (AG)n motifs and 5 of six (TC)n motifs gave polymorphic bands indicating that in sugarcane AG/TC is the most abundant. Primers based on (AC)n produced the highest level of polymorphism, followed by 5′ degenerate motifs and (GT)n motifs. PCR amplification using ISSR primers produced 9.5 bands, on average, for species amongst genus Saccharum, 11.6 for related genera and 6.6 within species. A similarity matrix and a dendrogram of 11 genotypes were generated, which were consistent with the result of Irvine. The characteristic features of the ISSR markers suggest their potential applicability to classification, phylogenetic analysis and construction of PCRbased genome maps in sugarcane.
【Fund】: 国家自然科学基金资助项目(30170590);; 863高新技术发展计划课题资助项目(2001AA241191).
【CateGory Index】: S566.1
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