Isolation of total RNA from tubers of Ipomoea batatas and cloning of conserving domain sequence of psy gene
CHEN Xuan-yang, YUAN Zhao-nian, ZHANG Zhao-juan, ZHENG Ya-feng, ZHENG Jin-gui (College of crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China)
The tubers of Ipomoea batatas are rich of secondary metabolites, which hinder the isolation of RNA. Four methods were used to isolate functional RNA from tubers of Ipomoea batatas, and the best one was screened out. Using the best method, high quality and yield of total RNA was obtained with no degradation or contaminant of complicated secondary metabolites such as starch, polysaccharides and phenolic compounds. The RNA was successfully used in reverse-transcription and isolation of 422 bp psy gene conserving sequence.