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《Journal of Fujian Agricultural and Forestry University(Natural Science Edition)》 2005-03
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rDNA ITS gene of silk sorghum by RFLP

GUO Qiong-xia~1,HUANG Ke-hui~1,YU Yun~1,WU Zhen-quan~2(1.Fujian Entry-Exit Inspection and Quarantine Bureau,Fuzhou,Fujian 350001,China;2.Bio-control Institute,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)  
Internal transcribed spacer(ITS) sequences of rDNA from silk sorghum as a quarantine weed and related species were obtained.By restriction endonuclease digestion,restriction enzyme XceⅠcut the PCR product.The sequence alignment showed that only 16 base pairs differences were found in ITS(ITS1,5.8S RNA,ITS2).It indicated that rDNA ITS region was relatively stable among the intrapopulations of 6 related species.The fragments of rDNA ITS-PCR were digested with XceⅠ.The difference between silk sorghum and its related species was found according to the pattern of digestion by XceⅠ.That is,only two bands in 670,190 bp were observed in the lanes of silk sorghum while the four fragments in 670,530,190 and 150 bp for other related species.The results were consistent with the fact that S.halepense,S.propinguum,S.propinguum(Mingfu No.1),S.almum and S.sudanense each had two selective identify sites of PmaCⅠ while S.silk had one site.Therefore,silk sorghum can be distinguished by the PCR-RFLP method established in this study.
【Fund】: 国家质量检验检疫监督总局2002年科技项目(IK2002059)
【CateGory Index】: S41
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