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《Journal of Fujian Agricultural and Forestry University(Natural Science Edition)》 2005-03
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rDNA ITS gene of silk sorghum by RFLP

GUO Qiong-xia~1,HUANG Ke-hui~1,YU Yun~1,WU Zhen-quan~2(1.Fujian Entry-Exit Inspection and Quarantine Bureau,Fuzhou,Fujian 350001,China;2.Bio-control Institute,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)  
Internal transcribed spacer(ITS) sequences of rDNA from silk sorghum as a quarantine weed and related species were obtained.By restriction endonuclease digestion,restriction enzyme XceⅠcut the PCR product.The sequence alignment showed that only 16 base pairs differences were found in ITS(ITS1,5.8S RNA,ITS2).It indicated that rDNA ITS region was relatively stable among the intrapopulations of 6 related species.The fragments of rDNA ITS-PCR were digested with XceⅠ.The difference between silk sorghum and its related species was found according to the pattern of digestion by XceⅠ.That is,only two bands in 670,190 bp were observed in the lanes of silk sorghum while the four fragments in 670,530,190 and 150 bp for other related species.The results were consistent with the fact that S.halepense,S.propinguum,S.propinguum(Mingfu No.1),S.almum and S.sudanense each had two selective identify sites of PmaCⅠ while S.silk had one site.Therefore,silk sorghum can be distinguished by the PCR-RFLP method established in this study.
【Fund】: 国家质量检验检疫监督总局2002年科技项目(IK2002059)
【CateGory Index】: S41
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【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Zhang Yun Wang Mingchang Li Yan Sui Na Dong Changyong(College of Life Sciences,Liaoning Normal University,Dalian 116029,Liaoning,China);Structural Character and Functional Evolution in rRBA Genes in Marine Animals[J];Anhui Agricultural Science Bulletin;2008-05
2 LIU Zhi-wen et al (Rice Institute,Shenyang Agricultural University,Shenyang,Liaoning 110161);Application of NuclearRibosomal DNA in the the Phylogenetic Development of Plants and Its Research Progresses[J];Journal of Anhui Agricultural Sciences;2008-09
3 LI Xue-ying,PENG Jian-ying,PENG Shi-qi(College of Horticulture,Agricultural University of Hebei,Baoding 071001,China);Comparison between extracting methods for genomic DNA of dry leaf of Ziziphus Mill.saved in silica[J];Journal of Agricultural University of Hebei;2006-01
4 Shi Quanliang(Biology Department,Suzhou University,Suzhou 215151)Zhao Weiguo(The Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang 212018);Preliminary Study on ITS Sequencing and Characteristics of Mulberry[J];Acta Sericologica Sinica;2001-02
5 ZHAO Weiguo PAN Yile ZHANG Zhifang(Key Laboratory of Silkworm Biotechnology of Ministry of Agriculture; The Sericultural Institute Research, Chinese Academy of Agricultural Sciences, Zhenjiang 212018);Phylogenetic Relationship of Genus Morus by ITS Sequence Data[J];Acta Sericologica Sinica;2004-01
6 WU Qi1,ZENG Zi-xian1,SHAO Ji-rong1,CHEN Hui1,TANG Yu2 (1.College of Life Science,Sichuan Agricultural University,Ya'an 625014,China;2.Sichuan Higher Institute of Cuisine,Chengdu 610072,China);Sequence analysis of internal transcribed spacer of Fagopyrum dibotrys and its systematic utility[J];Acta Prataculturae Sinica;2007-05
7 LI Xue-ying,PENG Jian-ying,BAI Rui-xia (College of Horticulture, Agricultural University of Hebei, Baoding , Hebei 071 001,China);Use of ITS Sequences Based on Nuclear rDNA for Phylogenetic Research of Seed Plants[J];Acta Botanica Boreali-occidentalia Sinica;2005-04
8 ZHANG Li1,2,ZHOU Yong-hong2*,DING Chun-bang1,2,YANG Ruiwu1,2,FAN Xing2(1 College of Biology and Science,Sichuan Agricultural University,Yaan,Sichuan 625014,China;2 Key Laboratory of Crop Genetic Resources and Improvement,Ministry of Education,Sichuan Agricultural University,Yaan,Sichuan 625014,China);The Phylogenetic Relationships of Kengyilia Species Based on Evidences from ITS Sequences[J];Acta Botanica Boreali-Occidentalia Sinica;2007-03
9 XIE Hui1, CHAO Zhi2, HUO Ke-ke3, WU Bing-yi4, PAN Sheng-li1 1School of Pharmacy, Fudan University, Shanghai 200032, China; 2School of Tradition Chinese Medicine, Southern Medical University, Guangzhou 510515, China; 3Institute of Biological Science, Fudan University, Shanghai 200433, China; 4Clinical Research Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China;ITS sequence of 9 Bupleurum species and its application in identification of Chaihu (Radix Buplurei)[J];Journal of Southern Medical University;2006-10
10 YANG Zhi-ye1,CHAO Zhi2,HUO Ke-ke3,WU Bing-yi4,PAN Sheng-li1 1School of Pharmacy,Fudan University,Shanghai 200032,China;School of Traditional Chinese Medicine2,Clinical Research Center,Nanfang Hospital4,Southern Medical University,Guangzhou 510515,China;3School of Life Science,Fudan University,Shanghai 200433,China;Nuclear ribosomal DNA internal transcribed spacer 1 sequences of 4 Leonurus species[J];Journal of Southern Medical University;2006-11
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 SHAO Bi-ying, CHEN Wen-bing, JIANG Shu-xun, LI Shou-song (Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou, Fujian 350003, China);Multiplex polymerase chain reaction(MPCR) analysis of genetically modified ingredients of the soybean and beanthread[J];;2004-01
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