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《Journal of Fujian Agriculture and Forestry University(Natural Science Edition)》 2006-03
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Optimization of the conditions for protoplast isolation from litchi transgenic embryogenic calli

HUANG Zhi-ying,LAI Zhong-xiong(Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)  
In this experiment the transgenic embryogenic calli with htp and gus were used as materials for optimizing the conditions for protoplast isolation in Litchi chimensis Soon.cv.Xiafanzhi.The results showed that litchi transgenic embryogenic calli after culture for 16-18 d were transferred into the CPW+130 g·L~(-1) mannital enzymatic solution containting 8 g·L~(-1) Cellulose R-10,4 g·L~(-1) Macrease R-10 continuously shaking at 30-40 r·min~(-1) for 10-11 h at(25±2) ℃ in darkness for innoculation;and the protoplast yield of 18.2×10~6·g~(-1) and viability of 97.5% were obtianed under the optimized procedures in the resistant cell line Q811.
【Fund】: 国家自然科学基金资助项目(39870544);; 霍英东高校青年教师基金资助项目(71026);; 福建省自然科学基金资助项目(C97030)
【CateGory Index】: S667.1
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