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《Journal of Fujian Agriculture and Forestry University(Natural Science Edition)》 2011-01
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ITS sequence analysis of intraspecific genetic diversity of Hypoderma rubi

LIU Fang1,LIN Ying-ren1,WANG Shi-juan1,GAO Xiao-ming2,CHEN Li1,JIA Guo-jun2(1.School of Forestry and Landscape Architecture;2.School of Life Science,Anhui Agricultural University,Hefei,Anhui 230036,China)  
Fresh materials of Hypoderma rubi collected from Anhui Province were isolated by tissue isolation technique,and 12 pure strains were obtained.DNAs of all the strains were extracted from the dry mycelia,rDNA-ITS regions were amplified by PCR and then sequenced.The ITS sequence analysis results showed that the length range of ITS sequences of H.rubi was from 443 to 456 bp,and the percentage of G+C contents of the ITS sequences varied from 48.7% to 51.5%.There were obvious variations in the ITS sequences of different strains and showed abundant genetic diversity among them.A phylogenetic tree was constructed by neighbour-joining method using MEGA 4.1.The results indicated that the intraspecific genetic diversity of H.rubi was related to hosts obviously,and strains from woody plants and herbaceous plants probably were two distant relationship groups.In addition,the intraspecific genetic differences of H.rubi were independent of parasitic sites and sample collection area.
【Fund】: 国家自然科学基金(30870014 30770006);; 教育部高校博士点专项科研基金(20070364002);; 安徽省自然科学基金(070411005)
【CateGory Index】: S436.639
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