Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Fujian Agriculture and Forestry University(Natural Science Edition)》 2011-01
Add to Favorite Get Latest Update

ITS sequence analysis of intraspecific genetic diversity of Hypoderma rubi

LIU Fang1,LIN Ying-ren1,WANG Shi-juan1,GAO Xiao-ming2,CHEN Li1,JIA Guo-jun2(1.School of Forestry and Landscape Architecture;2.School of Life Science,Anhui Agricultural University,Hefei,Anhui 230036,China)  
Fresh materials of Hypoderma rubi collected from Anhui Province were isolated by tissue isolation technique,and 12 pure strains were obtained.DNAs of all the strains were extracted from the dry mycelia,rDNA-ITS regions were amplified by PCR and then sequenced.The ITS sequence analysis results showed that the length range of ITS sequences of H.rubi was from 443 to 456 bp,and the percentage of G+C contents of the ITS sequences varied from 48.7% to 51.5%.There were obvious variations in the ITS sequences of different strains and showed abundant genetic diversity among them.A phylogenetic tree was constructed by neighbour-joining method using MEGA 4.1.The results indicated that the intraspecific genetic diversity of H.rubi was related to hosts obviously,and strains from woody plants and herbaceous plants probably were two distant relationship groups.In addition,the intraspecific genetic differences of H.rubi were independent of parasitic sites and sample collection area.
【Fund】: 国家自然科学基金(30870014 30770006);; 教育部高校博士点专项科研基金(20070364002);; 安徽省自然科学基金(070411005)
【CateGory Index】: S436.639
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 9 Hits
1 Zhu Heng Qu Feng Zhu Lihuang (Institute of Genetics, Academta Sinica, Beijing 100101);ISOLATION OF GENOMIC DNAS FROM FUNGI USING BENZYL CHLORIDE[J];Mycosystema;1994-01
2 Wang Hong-Kai Zhang Tian-Yu** Zhang Meng (Systematic Mycology and Lichenology laboratory, Chinese Academy of Sciences. Beijing 100080;Department of Plant Pathology, Shandong Agricultural University, Shandong 271018; Biotechnology Institute, Agricult;APPLICATION OF SEQUENCING OF 5.8S rDNA, ITS1 AND ITS2 ON IDENTIFICATION AND CLASSIFICATION OF ALTERNARIA AT SEPECIES LEVEL[J];Mycosystema;2001-02
3 CHEN Yong-Qing JIANG Zi-De QI Pei-Kun (College of Natural Resources & Environment, South China Agricultural University, Guangzhou, 510642);APPLICATION OF RAPD AND ITS REGION SEQUENCE ANALYSES ON CLASSIFICATION AND IDENTIFICATION OF PHOMOPSIS[J];Mycosystema;2002-01
4 CHEN Feng-mao College of Forest Resources and Environment,Nanjing Forestry University,210037,Nanjing,China;The Sequence of ITS Region and Its Application of Fungus[J];China Forestry Science and Technology;2007-02
5 Wang Yuanchao *,Ding Guoyun *,Ma Zhichao and Li Debao (Biotechnology Institute of Zhejiang University,Hangzhou 310029);Cloning and sequencing of ribosomal RNA gene ITS in Phytophthora boehmeriae and P.capasici[J];JOURNAL OF NANJING AGRICULTURAL UNIVERSITY;2000-03
6 YU Hong-mei1,LIN Ying-ren1,CHEN Li1,LIU Yan-bing2,Han Jia-jun2,GAO Xiao-ming2,GU Ting-ting1(1.Coll.of Forest & Garden;2.Coll.of Life Sci.,of Anhui Agric.Univ.,Hefei 230036);ISSR Analysis of Intraspecific and Interspecific Genetic Diversity of Hypoderma[J];Journal of Microbiology;2009-02
7 HE Yu-feng~1 GUI Xian-qun~2 YE Guang-bin~3 WANG Shi-juan~3 LIN Ying-ren~1 1.College of Forest and Gardening,Anhui Agricultural University,Hefei,Anhui 230036,China 2.National Forest Park of Tiantangzhai,Tiantangzhai,Anhui 237343,China 3.College of Life Science,Anhui Agricultural University,Hefei,Anhui 230036,China;RAPD Analysis of Intraspecific Heredity Difference for Hypoderma rubi[J];Journal of Fungal Research;2006-04
8 Zhu Youyong 1 Wang Yunyue 1 Bruce R. Lyon 2 (1 Phytopathology Lab. of Yunnan, Kunming 650201) (2 School of Biological Sciences. University of Sydney Australia. Sydney 2006);PCR DETECTION OF Verticillium dahliae FROM DISEASED PLANTS[J];ACTA PHYTOPATHOLOGICA SINICA;1999-03
9 YANG La-ying,HUANG Hua-ping,TANG Fu-run,HU Mei-jiao,ZHANG Shi-qing,HUANG Jun-sheng(Environmental and Plant Protection Institution,State Key Laboratory of Tropical Crop Biotechnology,Chinese Academy of Tropical Agricultural Science,Haikou 571101,China);Rapid molecular identification and detection of Colletotrichum musae with species-specific primers based on the internal transcribed spacer(ITS) region[J];Acta Phytopathologica Sinica;2006-03
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 TANG Jian-hui et al(State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237);Cloning and Sequencing of Ribosomal RNA Gene ITS in Colletotrichum orbiculare[J];Journal of Anhui Agricultural Sciences;2007-09
2 CHEN Jian-shan et al (Environment and Plant Protection College,SCUTA,Danzhou,Hainan 571737);Application of ITS Sequences in Fungi Classification and Identification[J];Journal of Anhui Agricultural Sciences;2007-13
3 ZHANG Chun-yang et al(School of Life Science,Shandong University of Technology,Zibo,Shangdong 255091);A New Species of Alternaria[J];Journal of Anhui Agricultural Sciences;2007-32
4 CHEN Yu-xi et al(College of Life Science,Hebei University,Baoding,Hebei 071002);Analysis of the Internal Transcribed Spacer(ITS) Sequences in rDNA of 10 Strains of Fusarium spp.[J];Journal of Anhui Agricultural Sciences;2008-12
5 SUN Wen-xiu et al(College of Life Science,Yangtze University,Jingzhou,Hubei 434025);Analysis of Genetic Diversity of Phytophthora capsici from Different Sources[J];Journal of Anhui Agricultural Sciences;2008-16
6 WANG Shi-juan et al(School of Forestry and Landscape Architecture,Anhui Agricultural University,Anhui,Hefei 230036);Biological Characteristics of the Needle Cast Pathogen of Lacebark Pine and ITS Sequence Analysis[J];Journal of Anhui Agricultural Sciences;2009-11
7 HAN Ji-yu et al (College of Animal Science and Veterinary Medicine,Inner Mongolia Agricultural University,Huhhot,Inner Mongolia 010018);Analysis on the Bacterial Diversity in the Dynamic Fermentation System of Corn and Alfalfa Silage by PCR-DGGE[J];Journal of Anhui Agricultural Sciences;2009-19
8 HUANG Bo,FAN Mei zhen,LI Zeng zhi (Research Institute of Economic Insects & Fungi, Anhui Agricultural University,Hefei 230036);Cloning and Sequencing of ITS1-5.8S-ITS2 rDNA Region of Paecilomyces farinosus and .tenuipes[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;2002-01
9 CHENG Guo-wang~1,WU Zhong-hua~2,XU Jian~2, GU Yun-hong~1, YU Zeng-liang~1 of Plasma Physics, Chinese Academy of Sciences,Hefei 230031; 2.Hefei Fengle Seed Co.Ltd.,Hefei 230031);Comparison and Improvement of the Methods of DNA Extraction from Mortierella alpina[J];Journal of Anhui Agricultural University;2004-02
10 ZHOU Quan~1,HUANG Bo~(1,2),LI Shi-gui~3,WANG Bin~1,HU Feng-lin~1,PENG Fan~1,LI Zeng-zhi~1 (1.Anhui Provincial Key Laboratory for Microbial Pest Control, Anhui Agricultural University, Hefei 230036; 2.Systematic Mycology & Lichenology Laboratory,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100080; 3.Institute of Soil and Fertilizer Sciences,Chinese Academy of Agricultural Sciences, Beijing 100081);Molecular Identification of the Metarhizium Strain (ACCC30104)[J];Journal of Anhui Agricultural University;2005-01
China Proceedings of conference Full-text Database 9 Hits
1 YU Xia~(1*) YANG Dan-Ling~2 CHEN Jin-Hui~3 GONG Guo-Shu~4 1.Heyuan Enter-exit Inspection and Quarantine, Heyuan 517000, China; 2.Chongqing,Dadukou District Party School,Chongqing 400084, China; 3.Dongguan Enter-exit Inspection and Quarantine, Dongguan 523072, China; 4.Agricultural College, SichuanAgricultural University, Yaan 625014, China;The Status of Classification and the Method of Identification for Fungi[A];[C];2008
2 Xie Bao-Gui Liu Wei-Xia Wang Xiu-Quan Jiang Yu-Ji Fujiang Agricultural and Forest University, Fuzhou, 350002;Study on Molecular Marker for Fruit-body color Gene of Flammulina velutipes[A];[C];2004
3 ZHENG Hua-ying1, QIAN Guo-liang2, XU Fu-yuan1, HU Bai-shi2, ZHANG Ya-chuan3, XIE Chun-xia1, XU Zhi-gang2(1.Jiangsu Academy of Forestry,Na ing,21 1 153,China;2.Department of Plant Protection,Nanjing Agricultural University,Nanjing 210095,China;3.Forest Disease&Insect Control and Quarantine Station of Huai'an City,Huai'an,223001,China);Identification and ITS sequence analysis of the canker disease of the new poplar forestation in north of Jiangsu province[A];[C];2009
4 Xu Jinzhu1,2, Huang Bo1, Qin Changsheng2 Li Zengzhi1 (1 .Research Center for Entomogenous Fungi, Anhui Agricultural University, 130 West Changjiang Rd, Hefei, Anhui, 230036, China;2. Guangdong Forestry Research Institute, 1 Guangshan Rd, Guangzhou, Guangdong, 510520, China);Colony sectorization analysis of Beauveria bassiana during subculturing[A];[C];2009
5 Zheng Huaying1, Qian Gu-liang2, Xu Fu-yuan1, Hu Bai-shi2, Zhang Yachuan3, Xie Chunxia1, Xu Zhi-gang2 (1. Jiangsu Academy of Forestry,Na ing,21 1 153,China;2.Department of Plant Protection,Nanjing Agricultural University, Nanjing 210095,China; 3.Forest Disease&Insect Control and Quarantine Station of Huai’an City,Huai’an,223001,China);Identification and ITS sequence analysis of the canker disease of the new poplar forestation in north of Jiangsu province[A];[C];2009
6 LI Shu-nuo~1,LI Ya-dong~(1*),and WANG Qi~2 (1 College of Horticulture,Jinlin Agricultural University,Changchun130118,China;2 College of Chinese Traditional Medicine,Jinlin Agricultural University,Changchun130118,China);Application of Sequencing ITS rDNA on Identifcation and Classification of Endophytic Fungi of Vaccinium at Species Level[A];[C];2009
7 YANG ZhongZhou~1 LIN YingRen~(1*) WANG ShiJuan~1 CHEN Li~3 GAO XiaoMing~2 ZHENG Qian~2 (1 School of Forestry & Landscape Architecture,Anhui Agricultural University,Hefei 230036,China;2 School of Life Sciences,Anhui Agricultural University,Hefei 230036,China; 3 College of Plant Protection,Anhui Agricultural University,Hefei 230036,China);Comparative studies of morphological characteristics and molecularity of Lophodermium pinastri and L.pini-excelsae[A];[C];2010
8 LIU Fang~1 CHEN JiangLin~2 LIN YingRen~(1,2*) WANG ShiJuan~1 GAO XiaoMing~2 CHEN Li~1 (1 School of Forestry and Landscape Architecture,Anhui Agricultural University,Hefei 230036, China;2 School of Life Science,Anhui Agricultural University,Hefei 230036,China);ITS sequence analysis of intraspecific genetic diversity of Hypoderma rubi[A];[C];2010
9 XU Feng,LIUYu,WANG Shouxian,ZHAO Shuang,WEI Min,GENG Xiaoli,MENG Lili (Institute of Plant and Environmental Protection,Beijing Academy of Agriculture and Forestry Science,Beijing 100097,China);A Simple Method for Extraction of Genomic DNA of Mushrooms[A];[C];2010
【Secondary Citations】
Chinese Journal Full-text Database 8 Hits
1 HUANG Xue-shun~(1)) FU Yan-ping ~(2)) QIN Le-ye~(3)) LI Xun-yuan~(1)) XIANG Qing-feng~(1)) ZENG Guo-zhu~(1)) JIANG Dao-hong~(2,4))(~(1))Extension Station of Agricultural Science and Technology at Duzhenwan,Hubei Province,Changyang 443509,China;~(2))College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;~(3))Agricultural Office of Changyang County,Hubei Province,Changyang 443500,China;~(4))State Key Lab of Agriculture Microbiology,Huazhong Agricultural University,Wuhan 430070,China);The Leaf Spot on Gardenia jasminoides and Identification of Its Pathogen[J];Journal of Huazhong Agricultural University(Natural Science Edition);2006-01
2 MA Zhi-gang LU Zuo-zhou ZHENG He-bin WANG Zhuo-ren(Institute of Applied Mycology,Huazhong Agricultural University,Wuhan 430070,China);The Prelimery Application of ISSR Marker in Taxonomy of Pleurotus Species[J];Journal of Huazhong Agricultural University(Natural Science Edition);2006-01
3 Zhu Heng Qu Feng Zhu Lihuang (Institute of Genetics, Academta Sinica, Beijing 100101);ISOLATION OF GENOMIC DNAS FROM FUNGI USING BENZYL CHLORIDE[J];Mycosystema;1994-01
4 CHEN Yong-Qing JIANG Zi-De QI Pei-Kun (College of Natural Resources & Environment, South China Agricultural University, Guangzhou, 510642);APPLICATION OF RAPD AND ITS REGION SEQUENCE ANALYSES ON CLASSIFICATION AND IDENTIFICATION OF PHOMOPSIS[J];Mycosystema;2002-01
5 SUN Li-Fu 1YANG Guo-Ting 1,2 QIN Guo-Fu 3SONG Yu-Shuang 3 SONG Rui-Qing 1(1. Northeast Forestr y University,Harbin150040)(2. Forestry Bureau of Heilongjiang Province, Harbin 150000)(3. Forest Plant Diseases and Insect Pests Control General Station, National Forestry General Bureau of China, Shenyang 110034);AN ATTEMPET ABOUT STUDIES ON THE PHYLOGENETICS OF ARMILLARIA GALLICA WITH ISSR MARKER[J];Bulletin of Botanical Research;2003-03
6 Deng Wei ( Physical Culture Department of Y A U, Kunming 650201 );The Current Situation Analysis of Students Figure and Exercise Function in Y A U[J];JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY;2000-02
7 YANG Peiwen, LI Jiarui, YANG Qinzhong, ZENG Li, WANG Qun( Plant Protection Institution of Yunnan Academy of Agricultural Sciences, Kunming 650205, China );Cloning and Sequencing the Internal Transcribed Spacer of the Ribosomal Gene of Plasmodiophora brassicae and Applying it in Detecting the Pathogen[J];Journal of Yunnan Agricultural University;2003-03
8 Wang Yuanchao Chen Qinghe Zheng Xiaobo Lu Jiayun (Department of Plant Protection ^Nanjing Agricultural University,Nanjing);INHIBITION OF METALAXYL ON THE OOSPORE PRODUCTION OF PHYTOPHTHORA BOEHMERIAE[J];Journal of Plant Protection;1997-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved