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Determination of Rice ragged stunt virus and vector transmission characteristics

ZHANG Song-bai1,2,SONG Guo-wei1,YANG Liang1,WU Zu-jian1,XIE Lian-hui1(1.Key Laboratory of Plant Virology of Fujian Province/Institute of Plant Virology,Fujian Agriculture and Forestry University, Fuzhou,Fujian 350002,China;2.College of Agriculture,Yangtze University,Jingzhou,Hubei 434025,China)  
RT-PCR and viral dsRNA identification assays were established to detect Rice ragged stunt virus(RRSV) in Nilaparvata lugens and rice plants,and vector transmission characteristics were analyzed using artificial inoculation.The results showed that viral dsRNA identification assay could detect virus from rice samples as less as 0.5 g,and RT-PCR assay was suitable for detecting RRSV in individual brown planthopper.The viruliferous ratios of nymphs and adults of N.lugens inoculated by RRSV were 68.2% and 75.0%,respectively.50.0% of nymphs and 32.5% of adults could transmit RRSV after virus acquisition.These results revealed that the brown planthopper population had high compatibility to RRSV with strong capacity to transmit the virus.
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