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《JOURNAL OF FUJIAN AGRICULTURAL UNIVERSITY》 1997-02
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Induction of litchi embryogenic calli by immature embryos and anthers culture in vitro

Yu Changhe Chen Zhenguang (Department of Horticulture, FAU, Fuzhou 350002)  
Immature embryos of Yuanhong, one of major litchi (Litchi chinensis Sonn.) cultivars in Fujian, were used as explants to optimizing media for inducing embryogenic callus (EC). Sucrose at 50 g·L -1 and 2,4 D at 2 ̄4 mg·L -1 had favorable effect on inducing EC in comparison with sucrose at 30 g·L -1 and 2,4 D at 0. 5 ̄ 1.0 mg·L -1 . The presence of active carbon at 5 g·L -1 or BA 0.5 mg·L -1 decreased percentage of EC. However, supplementing STS (29.[KG-4]4 μmol·L -1 ) could counteract the prohibitory effect of BA. On MS basal medium supplemented with 2,4 D at 2 mg·L -1 and sucrose at 50 g·L -1 , ECs were induced from immature embryos of litchi cultivars Xiaofanzhi, Wuye, Chenzi and Yuanhong with a frequency of 22%, 14.5%, 17.2% and 16.8%, respectively. EC was also induced from anthers of Yuanhong on the medium above with a frequency of 0.1% ̄0.6% and the presence of STS in this medium enhenced the frequency to 1.2% ̄5.6%. Discussions were made about effect of these factors and their interactions on the induction of EC.
【CateGory Index】: S667.1
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