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Induction of litchi embryogenic calli by immature embryos and anthers culture in vitro

Yu Changhe Chen Zhenguang (Department of Horticulture, FAU, Fuzhou 350002)  
Immature embryos of Yuanhong, one of major litchi (Litchi chinensis Sonn.) cultivars in Fujian, were used as explants to optimizing media for inducing embryogenic callus (EC). Sucrose at 50 g·L -1 and 2,4 D at 2 ̄4 mg·L -1 had favorable effect on inducing EC in comparison with sucrose at 30 g·L -1 and 2,4 D at 0. 5 ̄ 1.0 mg·L -1 . The presence of active carbon at 5 g·L -1 or BA 0.5 mg·L -1 decreased percentage of EC. However, supplementing STS (29.[KG-4]4 μmol·L -1 ) could counteract the prohibitory effect of BA. On MS basal medium supplemented with 2,4 D at 2 mg·L -1 and sucrose at 50 g·L -1 , ECs were induced from immature embryos of litchi cultivars Xiaofanzhi, Wuye, Chenzi and Yuanhong with a frequency of 22%, 14.5%, 17.2% and 16.8%, respectively. EC was also induced from anthers of Yuanhong on the medium above with a frequency of 0.1% ̄0.6% and the presence of STS in this medium enhenced the frequency to 1.2% ̄5.6%. Discussions were made about effect of these factors and their interactions on the induction of EC.
【CateGory Index】: S667.1
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3 XU Shan-shan et al.(Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002);Optimization of in vitro Minimal Growth Conservation Conditions of Embryogenic Callus in Ancient Litchi Trees[J];园艺与种苗;2013-05
4 WANG Guo1,2,LI Huanling2,WANG Jiabao2 1 College of Agriculture,Hainan University,Haikou,Hainan 570228,China 2 Environments and Plant Protection Institute,Chinese Academy of Tropical Agriculture Sciences,Haikou,Hainan 571101,China;Callus Induction from the Anthers of Two Litchi (Litchi chinensis Sonn.)Cultivars[J];热带作物学报;2013-04
5 GUO Shao-yun1,PENG Hong-xiang2,DING Feng1,LI Hong-li2,QIN Xian-quan2,XU Ning2,HE Xin-hua1(1.College of Agriculture,Guangxi University,Nanning 530004,Guangxi China;2.Horticulture Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,Guangxi China);A Review of Advances in Genetic Transformation System Research on Litchi and Longan[J];亚热带植物科学;2012-04
6 WANG Zi-qing1,2,LIU Ai-ping3,HU Xiao-yuan4,WANG Jia-fu5,(1.Agricultural Comprehensive Development Office of Weihai City,Shandong Province,Weihai 264200,China;2.College of Plant Protection,Fujian Agricultural and Forestry University,Fuzhou 350002,China;3.Country Committee of Wenfeng District of Anyang City,He'nan Province,Anyang 455000,China;4.Forestry Bureau of Jiaxiang County of Shandong Province,Jiaxiang 272400,China;5.Institute of Subtropical Fruits,Fujian Agricultural and Forestry University,Fuzhou 350002,China),;Induction and screening of in vitro conservation conditions of embryogenic callus of Litchi chinensis[J];植物资源与环境学报;2009-01
7 ZHOU Xu-hong,MO Xi-jun,WU Ming,GUI Min (Flower Research Institute,Yunnan Academy of Agricultural Sciences,Kunming 650205,China);Research Progress in Anther Culture[J];江西农业学报;2007-08
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10 Huang Suhua1,2 Lai Zhongxiong21 Institute of Subtropical Fruits, Fujian Agriculture and Forestry University Fuzhou Fujian 350002 China 2 Department of Biology, Longyan College, Longyan Fujian Fujian 364000 China;Genomic DNA Extraltion of Cultures and RAPD Analysis in the Process of Somatic Embryogenesis in Litchi[J];热带作物学报;2006-04
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1 CHE Jianmei;LAI Zhongxiong;Synchronization of early somatic embryogenesis and somatic embryogenesis at high frequency from embryogenic callus in litchi[A];[C];2003
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3 DING Feng1,PENG Hong-xiang2,LI Hong-li2,ZHU Jian-hua2,QIN Xian-quan2,SHEN Qing-qing1,HE Xin-hua1(1.Colege of Agriculture,Guangxi University,Nanning 530004,Guangxi China;2.Horticulture Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,Guangxi China);Research Advances in AP1 Genes of Plants[J];亚热带植物科学;2011-01
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5 GAO Yu-qiong,LAI Zhong-xiong(Institute of Horticultural Biotehnology,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China);A study on callus culture from somatic embryos and leaves in Camellia nitidissima Chi.[J];亚热带农业研究;2010-02
6 Luo Cong 1 He Xinhua 1,2 Chen Hu 1 Jiang Yaqin 1 Gao Meiping 1 Li Yangrui 2 1 College of Agriculture, Guangxi University, Nanning, 530004; 2 Guangxi Crop Genetic Improvement and Biotechnology Lab, Nanning, 530007;Cloning and Bioinformatic Analysis of the AP1 Homolog Gene from Mango[J];基因组学与应用生物学;2009-05
7 Lin Xiulian,Lai Zhongxiong Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University;Fujian In-vitro Bank of Germplasm of Fujian and Taiwan Fruits,Fuzhou,Fujian 350002;Minimal Growth Conservation and Variation of Chromosome Number of Longan Embryogenic Callus[J];热带作物学报;2009-10
8 LI Wei-ming1,2,WANG Shuang-shuang1,YAO Yu-xin1,ZHAO Chang-zeng2,HAO Yu-jin1,and YOU Chun-xiang1(1State Key Laboratory of Crop Biology,College of Horticulture Science and Engineering,Shandong Agricultural University,Tai’an,Shandong 271018,China;2Department of Horticulture,College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China);Genetic Transformation of Apple MdFT Gene into Tomato[J];园艺学报;2009-09
9 CHEN Lin-jing1,FENG Hui2,CONG Ri-chen2,et al.(1.College of Biologe,Jinzhong University,Yuci Shanxi 030600,China;2.Beijing Institute of Landscape Gardening,Beijing 100102,China);Establishment of Plant Regeneration from Anther of Petunia Hybrida[J];山西农业大学学报(自然科学版);2009-04
10 Guo Chunxiao Tian Subo Zheng Chengshu Wang Wenli Sun Xianzhi College of Horticulture Science and Engineering, Shandong Agricultural University, State Key Laboratory of Crop Biology, Taian, 271018;FT Gene of Flowering Determination of Plants in Photoperiod Pathway[J];基因组学与应用生物学;2009-03
【Secondary References】
Chinese Journal Full-text Database 9 Hits
1 Fan Jianxin;Deng Renju;Wang Yongqing;Liu Rong;Han Shuquan;Zhu Anle;College of Horticulture,Sichuan Agricultural University;Guizhou Institute of Subtropical Crops;Guizhou Biotechnological Institute;Agriculture Bureau of Guanling Autonomous County;;Morphological Characteristics of Pitaya Microspore at Different Developmental Stages[J];分子植物育种;2017-02
2 Fan Jianxin;Deng Renju;Wang Yongqing;Liu Hong;Peng Zhijun;Liu Tao;College of Horticulture, Sichuan Agricultural University;Guizhou Institute of Subtropical Crops;Guizhou Biotechnological Institute;Guizhou Fruits Institute;;Embryogenic Callus Induction Via Anther Culture of Purple Pitaya (Hylocereus costaricensis cv. Zihonglong) and their Genetic Stability[J];分子植物育种;2017-01
3 Wang Honggang;Li Ye;Chen Chengbin;Wang Chunguo;Chen Li;Song Wenqin;Bai Yanling;Zhang Jinhong;Liu Fang;Biological ExperimentalTeaching Center,Nankai University;;Research on experiment of cell fusion induced by electric shock[J];实验技术与管理;2017-01
4 HUANG Cui-hong;YANG Gui-li;HUANG Ming;ZHOU Dan-hua;LIU Yong-zhu;GUO Tao;CHEN Zhi-qiang;WANG Hui;National Engineering Research Center of Plant Space Breeding,South China Agricultural University;;Primary research on anther culture of photo-thermosensitive genic male sterile rice “Y58S” and selection of descendant of anther culture[J];广东农业科学;2016-05
5 LI Ping;PENG Jing-ru;HUANG Qiu-wei;LI Hui-min;SU Wen-pan;Guangxi Zhuang Autonomous Region Research Institute for Subtropical Crops;;Effects of Culture Conditions on SOD Activity of Embryonic Callus in Cassava[J];湖北农业科学;2015-08
6 He Dan;Liu Yannan;Wang Zheng;Wang Zhaolu;He Songlin;College of Forestry,Henan Agricultural University;;Effects of Exogenous Hormones in the Process of Embryogenic Callus Induction in Tree Peony[J];河南科学;2015-03
7 DONG Huixue;ZHOU Yanrong;TIAN Qilin;LAI Gongti;LIN Yuling;LAI Zhongxiong;Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University;;Effects of Different Light Qualities on the Contents of Flavonoids of Embryonic Callus in Dimocarpus longan Lour.[J];热带作物学报;2014-12
8 HONG Sen-Rong;YIN Ming-Hua;WANG Ai-Ping;College of Life Sciences,Shangrao Normal University;;Cryopreservation of Jiangxi Yanshan red bud taro(Colocasia esculenta var. cormosus cv. Hongyayu)embryogenic callus by vitrification and its plantlet regeneration[J];广西植物;2014-03
9 GUO Shao-yun;PENG Hong-xiang;HE Xin-hua;DING Feng;LI Hong-li;QIN Xian-qun;XU Ning;College of Agriculture,Guangxi University;Horticulture Research Institute,Guangxi Academy of Agricultural Sciences;Guangxi Crop Genetic Improvement Laboratory;;Callus Induction from Different Explants of Litchi[J];西南农业学报;2014-02
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