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《Fujian Journal of Agricultural Sciences》 2008-02
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Genetic diversity among population of Phytophthora infestans in Fujian province

L Xin1,2,3,LAN Cheng-zhong1,LI Ben-jin1,CHEN Qing-he1(1.Institute of Plant Protection,Fujian Academy of Agricultural Science,Fuzhou,Fujian 350013,China;2.Central Laboratory,Fujian Academy of Agricultural Science,Fuzhou,Fujian 350003,China;3.Key Public Precision Testing Laboratory for Agricultural Applications,Fuzhou,Fujian 350003,China)  
Genomic fingerprints of 63 strains of the pathogen,Phytophthora infestans,from different areas and hosts were analyzed by using 12 RAPD primers.These primers were randomly selected from 150 primers with abundant polymorphisms.Genomic DNA from the different strains were amplified with the 12 selected primers resulting in 92 marked bands.Among the bands,85 were polymorphic,which accounted for 92.4% of the total bands produced.Based on the genetic distance,a dendrogram was constructed with the software NTSYSpc Version 2.1.Of the 63 strains tested,6 genetic groups were formed.There was no correlation found between RAPD group and the geographic origin or their host.The analysis showed that some strains from one area were closely related to some from another area.Nonetheless,rich genetic variation existed among the tested strains.Migration and A2-type mating of the late blight fungus might have been the cause of the diversity.
【Fund】: 国家自然科学基金项目(30600401);; 福建省自然科学基金项目(2006J0322);; 福建省财政专项(STIF-Y07);; 农业部公益性行业(农业)科研专项(nyhyzx07-053)
【CateGory Index】: S432.44
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