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《Fujian Journal of Agricultural Sciences》 2011-03
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The Research of Highly Pathogenic PRRSV Distribution in Challenged Pigs

FANG Gui-you1,WANG Long-bai1,ZHUANG Xiang-sheng1,SHAO Liang-ping2,ZHOU Lun-jiang1,CHE Yong-liang1,WEI Hong1,CHEN Ru-jing1(1.Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China;2.College of Animal Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)  
In order to understand the distribution of highly pathogenic PRRSV in challenged pigs.Quantity and distribution of highly pathogenic PRRSV in different tissues of pigs challenged by highly pathogenic PRRSV were detected by the TaqMan RT-PCR.5 pigs of 35 days old were selected for this study,which were negative of PRRSV antibody.Among them,3 pigs were challenged by highly pathogenic PRRSV,one pig was cohabited,and another one was solitary as control.Clinical findings of all the pigs were recorded after challenged.All pigs were slaughtered at the 14th day of challenge and 14 tissue samples were collected.The results showed that all the samples were invaded by the virus,but the virus titers were higher in heart,lung,lymph nodes,tonsils,brain and blood than in other tissues.No positive result was obtained in the samples from the solitary pig.The results revealed the distribution of the highly pathogenic PRRSV in different organs and organization after challenged pigs.This research provided a scientific reference for epidemiology and pathogenesis of the highly pathogenic PRRSV.
【Fund】: 福建省自然科学基金项目(2009J06012);; 福建省财政专项——福建省农业科学院科技创新团队建设项目(STIF-Y02)
【CateGory Index】: S858.28
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