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《Journal of Fujian Teachers University(Natural Science)》 2000-04
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Purification and Characterization of a Lectin from Variety of Ipomoea Batatas

LIN Xiao hong 1,YU Ping 2,LIN Shao qin 2,LIN Xi 2 (1 Fujian Huanan Women's College, Fuzhou 350007,China; 2 Fujian Teachers University, Fuzhou 350007,China)  
The extraction solution of the variety of Ipomoea Batatas was graded by ammonium sulfate. It was affinity chromatographied by the chitin and filtered by molecular sieve. Author obtained the IBL (Ipomoea Batatas Lectin) which showed a single band on PAGE. And by SDS PAGE author measured subunit relative molecular mass to be about 47KD. IBL had agglutination on blood cells of rabbits, its agglutination activity was stopped by D fructose. IBL was heat unstable and lost all activity at 70℃. It was sensitive to alkali. IBL contained 5 07% neutral sugar and 16 kinds of amino acid and was rich in Asp and Glu.
【Fund】: 福建省自然科学基金资助项目!(C9810 0 12 )
【CateGory Index】: Q946
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