Study on the Anti-S_(180) Sarcoma of Nerium indicum Mill.Polysaccharide in Mice
WU Jian-zhang,XIAO Yi-jun,CHEN Bing-hua,ZENG Hong(College of Life Sciences,Fujian Normal University,Fuzhou 350108,China)
Polysaccharide from the leave of Nerium indicum Mill. was extracted by water and precipitated by ethanol,the mice S180 solid tumor and ascites tumor model was established to evaluate the anti-tumor activity of NP,the changes of expression of PCNA,bcl-2 and bax protein in tumor tissues were detected by immunohistochemical method. Enhancement of CTX anti-tumor effects by NP was explored further by coefficient of drug interaction. The results demonstrated that the content of polysaccharide is 81.14% determined by phenol-sulfuric acid method analysis. NP inhibited S180 sarcoma growth in mice and prolonged the life-span of S180-ascites tumor mice evidently. The tumor inhibition rates (IR) were 40.00%,50.83%,44.17% and the life-span extension rates (ER)were 42.86%,64.29%,43.88% after 50,100,150 mg/kg NP-ip . The positive rate of PCNA,bcl-2 in tumor tissues were decreased,and bax were enhanced remarkably in S180 solid tumor-bearing mice by NP treated. There was synergistic effect in the cooperation of NP and CTX.