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Study On Tissue Culture of Anoectochilus roxburghii

Fan Zinan Xiao Huashan Fan Xiaohong Wu Wengshan (Bioengineering college)  
the rapid propagation of Anoectochilus roxburghii by plant tissue culture was reported in this paper.The technological process of factorial scale for producing plantlet has been preliminary completed in A roxburghii . The experimental results indicated that at the best collecting the explant was conducted in May and the pH value of medium was kept in 5 4~5 6.The plant growth substance played an impotrtant part in the tissue culture of A.roxburghii .The different plant growth substance and their concentrations could induct the stem part of A.roxburghii to either plantlet once through or sprouting.It would take better effects on inducting of root when the medium supplemented with absorbent carbon or replaced agar by vermiculite.The survival rate of transplantation would raise along with increasing plantlet height.
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