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Studies on Soluble Polysaccharide by Acid Extraction from Dictyophora duplicata——Isolation,Purification and Identification of Dd—2DE

Lin Yuman(Experiment Center) Yan Chunsheng(Department of Physical Education) Yu Ping(Institute of Polymer Science)  
The watersoluble polysaccharide Dd-2DE was extracted by 3% trichloroacetic acid from dried fruit bodies of dictyohora duplicata and further graded and purified.Dd-2DE was proved homogeneous by chromatography on Sepharose 4B column and polyacrylamide gel electrophoreses.Dd—2DE contains D-Glue,D-Gal,D-Man,L-Fun and D-Xyl in the molar ratio of 047∶172∶100∶018∶021.Its molecular weight is about 76000 Da.
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