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Cultivation of Mouse Brain Microvascular Endothelial Cells in Vitro

XU Jian\|wen, WANG Wei, KANG Zhong\|han, et al (Department of Anatomy, Fujian Medical University, Fuzhou\ 350004, China)\;  
Objective\ To study the method of cultivation and to observe the ultrastructure characteristics of mouse brain microvascular endothelial cells.\ Furthermore,the activity of tissue\|type plasminogen activator(TPA) was detected.\ Methods\ Primary culture brain microvascular endothelial cells isolated from mouse brain were prepared by homogenate,filtration,collagenase digestion,differential conglutination.\ Cells from a confluent cell monolayer digested by 0.125% trypsin\|0.02% EDTA were used for passage.\ The activity of TPA was detected by using enzyme\|linked immunosorbent assays(ELISA).\ Results\ It was verified that cultivated cells synthesized and excreted TPA were vascular endothelial cells by morphology,ultrastructures and immunohistochemistry studies.\ Conclusion\ This results would provide a useful tool for studying diseases related to cerebral vessels in vitro.\;
【CateGory Index】: R329
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