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Expression of ET-1 mRNA and bcl-2 in Lung Injury Induced by Endotoxin Shock and the Protective Effect of Anisodamine or Dexamethasone

CHEN Hui, LIN Jian\|dong, LIN Cai\|zhu, et al (Department of Anesthesiology, The First Affiliated Hospital, Fujian Medical University, Fuzhou\ 350005, China)\;  
Objective\ To study the role of ET\|1 mRNA and bcl\|2 and the protective effect of anisodamine and dexamethasone in lung injury induced by endotoxic shock.\ Methods\ Twenty\|four SD mice were randomly divided into four groups(each group n=6).\ In group Ⅰ and Ⅱ,saline or lipopolysacharide(LPS) 5 mg/kg was administrated intravenously.\ In group Ⅲ and Ⅳ,anisodamine or dexamethasone was administrated at half an hour before LPS administration.\ The expression of ET\|1 mRNA and bcl\|2 of the lung tissue after five hours were analyzed by RT\|PCR and immunohistochemical method.\ Results\ Expression of ET\|1 mRNA in lung tissue of group Ⅱ was enhanced significantly compared with groupⅠ,Ⅲ,Ⅳ(P0 01).\ There is no significant difference in group Ⅲ,Ⅳ compared with group Ⅰ.\ Percent rate of cells with bcl\|2 expression increased markedly in group Ⅲ,Ⅳ,and only slightly in group Ⅱ.\ There was no expression in groupⅠ.\ Conclusion\ ET\|1 mRNA and bcl\|2 may induce lung injury in endotoxic shock.\ Anisodamine and dexamethasone could protect lung injury induced by endotoxic shock,and its mechanism might involve ET\|1 mRNA inhibition and bcl\|2 enhancement.\;
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