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《Journal of Fujian Medical University》 2005-01
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Determination of Aloe-Emodin and Aloin without Separation by Dual-Wavelength Spectrophotometry

HUANG Li-ying, LIN Xin-hua, LAI Li-Min, ZHENG Yan-jie, LIU Si-yi College of Pharmacy, Fujian Medical University, Fuzhou 350004, China  
Objective To develope a method for determination of Aloe-Emodin and also Aloin in Aloe without separation dual-wavelengths UV spectrophotometry. Methods Wavelengths were selected at 255 nm and 355 nm, and the reference wavelengths at 376 nm and 475 nm for Aloe-Emodin and Aloin respectively.Results The linearity correction of content and optics density in Aloe-Emodin was 2.00×10 -6~5.00×10 -5mol/L (r=0.9995) and that in Aloin was 2.00×10 -6~6.00×10 -5mol/L (r=0.9998). Conclusion This method is simple, convenient, accurate and could be used for the determination of Aloe-Emodin and also Aloin in Aloe sample.
【Fund】: 福建省科技厅重点资助项目 (2 0 0 2 I0 15 )
【CateGory Index】: R284.1
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