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《Journal of Fujian Medical University》 2006-04
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Rapid Determination of Chlorogenic Acid in Flowers of Lonicera japonica

Zhang Qiufang,Liu Bo,Shi Huai,Huang Sufang Biotechnology Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350003, China  
Objective To establish the method of rapid determination of chlorogenic acid in flowers of Lonicera japonica.Methods According to the color reaction,which was the specific color reaction occurring in FeCl_3 and phenolic hydroxide group,the reacting conditions were tested,such as wavelength,stability,standard operation curve and recovery rate so on.Results 755 nm was chosen as the detective wavelength.The regression equation was Y[WTBZ](μg/mL)=231.77X+5.4114,R~2=0.9982.The molar absorptive index was 1.495×10~3 L·mol~(-1)·cm~(-1).The detective limit was 3.78 μg/mL and the recovery rate was 92.22%.Conclusion The simple,rapid and low-cost method can be applied to evaluate the quality of Lonicera japonica in marketing.
【Fund】: 福建省自然科学基金资助项目(B0410024)
【CateGory Index】: R284
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