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《JOURNAL OF FUJIAN MEDICAL UNIVERSITY》 1999-02
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Factors Affecting the Formation of Protoplasts from Streptomyces hygroscopicus FC904

Qiang Hua 1, Huang Jie 2 (1. Department of Microbiology, Fujian Medical University, Fuzhou, 350004 (2. Fujian Institute of Microbiology, Fuzhou, 350007)  
Objective\ To study the factors affecting the formation of protoplasts from Streptomyces.hygroscopic(S. hygroscopicus) FC904.\ Methods\ Mycelia of S. hygroscopicus FC904 were treated with lysozyme and the numbers of protoplasts under different conditions were compared.\ Results\ The numbers of protoplasts under different conditions were different.\ The optimal conditions for the formation of the protoplasts are:(1)Media for mycelia growth should be supplemented with 0 7% glycine and 10% sucrose.\ (2)Mycelia used for preparing protoplasts should be acquired from mycelia in exponential phase.\ (3)Optimal concentration of lysozyme is 2 mg/ml.\ (4)Favorable lytic temperature is 30 ̄37℃.\ (5) Before digesting,1 ml mycelia debris should be suspended in 5 ml P buffer.\ Conclusion\ Component of the media for mycelia growth, growth phase of the mycelia, lysozyme concentration and lytic temperature are the main factors affecting the formation of protoplast from S. hygroscopicus FC904.
【CateGory Index】: TQ920.1
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