Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
Add to Favorite Get Latest Update

Factors Affecting the Formation of Protoplasts from Streptomyces hygroscopicus FC904

Qiang Hua 1, Huang Jie 2 (1. Department of Microbiology, Fujian Medical University, Fuzhou, 350004 (2. Fujian Institute of Microbiology, Fuzhou, 350007)  
Objective\ To study the factors affecting the formation of protoplasts from Streptomyces.hygroscopic(S. hygroscopicus) FC904.\ Methods\ Mycelia of S. hygroscopicus FC904 were treated with lysozyme and the numbers of protoplasts under different conditions were compared.\ Results\ The numbers of protoplasts under different conditions were different.\ The optimal conditions for the formation of the protoplasts are:(1)Media for mycelia growth should be supplemented with 0 7% glycine and 10% sucrose.\ (2)Mycelia used for preparing protoplasts should be acquired from mycelia in exponential phase.\ (3)Optimal concentration of lysozyme is 2 mg/ml.\ (4)Favorable lytic temperature is 30 ̄37℃.\ (5) Before digesting,1 ml mycelia debris should be suspended in 5 ml P buffer.\ Conclusion\ Component of the media for mycelia growth, growth phase of the mycelia, lysozyme concentration and lytic temperature are the main factors affecting the formation of protoplast from S. hygroscopicus FC904.
【CateGory Index】: TQ920.1
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 1 Hits
1 Qiang Hua\+1, Huang Jie\+2, Chen Yikai\+3,et al (1 Department of Microbiology, Fujian Medical University, Fuzhou,350004 2 Fujian Institute of Microbiology, Fuzhou, 350007 3.Laboratory of Genetic Engineering, Fujian Medical University, Fuzhou, 35000;Effects on the Production of Rapamycin by the Treatment of Protoplasts of Streptomyces hygroscopicus FC904 with Antibiotics and Nitrous Guanidine[J];JOURNAL OF FUJIAN MEDICAL UNIVERSITY;1999-03
Chinese Journal Full-text Database 2 Hits
1 XU XIAO-XUE,ZHANG TING-LAN AND ZHENG YOU-XIA (Shanghai Institute of Plant Physiology,Academia Sinica);FACTORS AFFECTING PROTOPLAST FORMATION AND REGENERATION OF STREPTOMYCETES[J];Acta Biologiae Experimentalis Sinica;1984-01
Chinese Journal Full-text Database 10 Hits
1 LIN Ying et al(College of Life Science,Shenyang Agricultural University,Shenyang,Liaoning 110161);Study on Protoplast Formation Condition of Trichoderma[J];Journal of Anhui Agricultural Sciences;2005-12
2 Qiang Hua 1, Chen Wenlie 2, Zhong Xiurong 2, et al (1.Departement of Microbiology,\ 2.Laboratory of Electron Microscopy, Fujian Medical University, Fuzhou, 350004);Observations on the Formation and Release of the Protoplasts from Streptomyces hygroscopicus FC904[J];JOURNAL OF FUJIAN MEDICAL UNIVERSITY;1999-01
3 Gong Shu-li, Deng Fang-ming, Chen Li-li (College of Food science and technology Hunan Agriculture University, Changsha 410128,China );Preparation of Protoplast of Flammulina Velutipes[J];Modern Food Science and Technology;2007-04
4 He Xiaorong; Li Yongquan;Zhao Xiaoli; Liu Huibin; Zhou Hongjun(College of Life Science,Hangzhou University,Hangzhou 310012);Selection of High-Yielding Demethylchlorotetracycline Mutant Strain Using Protoplast Mutagenesls[J];JOURNAL OF HANGZHOU UNIVERSITY(NATURE SCIENCE);1997-02
5 DONG Hong-Ping YUAN Sheng XU Xu-Shi XIN Ding-Kun (Faculty of Life Science, Nanjing Normal University, Jiangsu 210097);PROTOPLAST PREPARATION FORM DIASPORANGIUM SP.[J];Mycosystema;2001-04
6 Zhang Yuzhen, Ma Guirong and Gao Peiji (Inst. of Microbiol., Shandong Univ., Jinan);STUDIES ON THE FORMATION AND REGENERATION OF PROTOPLAT IN PENICILLIUM DECUMBENS[J];Journal of Shandong University;1989-02
7 Chen Xiaoge;Huang Ruishan;Zhu Zhangyu;Studies on the isolation and Regeneration of Protoplasts from Basidiomycetes[J];JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY;1994-02
8 Zheng Youxia Xu Xiaoxue(Shanghai Institute of Plant Physiology, Academia Sinica, Shanghai) Yang Pei Yang Lingzen Zu Wanlan(Shanghai No.4 Pharmaceutical Plant, Shanghai);STUDIES ON PROTOPLAST FORMATION,REGENERATION AND FUSED RECOMBINATION OF GENTAMYCIQN PRODUCER MICROMONOSPORA ECHINOSPORA[J];Chinese Journal of Biotechnology;1987-02
9 CHEN Li-li,MA Mei-hu,ZHOU Chuan-yun,GAO Bi-da(College of Food Science and Technology,Hunan Agricultural University,Changsha 410128,China);Effects of Different Factors on the Protoplast Preparation and Reproduction in Flammulina Velutipes[J];Biotechnology;2005-05
10 YANG Feng-ling,JIA Le,REN Xuan(College of Life Science,Shandong Agriculture University,Tai'an 271018,China);Protoplast Preparation and Regeneration of Pleurotus cornucopiae[J];Biotechnology;2007-06
Chinese Journal Full-text Database 10 Hits
1 Liu Daqun; Yang Wenxiang; Qi Bishu ;Wang Chaohua (Department of Plant Pathology, Hebei Agricultural University, Baoding 071001);Effect of Men-myco-93-63 and Its Fermentation Liquid on the Growth of Verticillium dahliae Strains of Cotton[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;1999-04
2 ZHANG Yan,MENG Qing-fang,YANG Wen-xiang,LIU Da-qun,LI Ya-ning,ZHANG Ting,NIU Kun (College of Plant Protection,Agricultural University of Hebei,Biological Control Centre of Plant Pathogens and Plant Pests of Hebei Province,Baoding 071001,China);The medium of Streptomyces roseoflavus Men-myco-93-63[J];Journal of Agricultural University of Hebei;2006-02
3 QIU Li 1, MENG Chun 1, GUO Yang-hao 1, HU Shi-guo 1, LING Ran 1, CHENG Shao-jun 2 (1. College of Qiaoxin Light Industry, Fuzhou University,Fuzhou, Fujian 350002, China; 2. Pharmaceutic School of Jiangxi Province, Nanchang, Jiangxi 330001, Chi;Production and regeneration of protoplast of streptomyces aureofaciens[J];Journal of Fuzhou University(Natural Sciences Edtion);2001-02
4 CHENG Ji1, HONG Wen-rong1, SU Jian-zhang1, YE Li-rong2 (1. College of Qiaoxing Light Industry, Fuzhou University, Fuzhou, Fujian 350002, China; 2. Fuzhou Antibiotic General Factory, Fuzhou, Fujian 350002, China);Study on protoplast fusion between Streptomyces tenebrarius and Streptomyces kanamyceticus[J];Journal of Fuzhou University(Natural Sciences Edtion);2003-01
5 CHEN-Peng 1,CHEN Chong-hong 1, CHENG Yuan-rong 2(1.Department of Pharmacy, Fujian Medical University, Fuzhou 350004;China;2.Fujian Institute of Microbiology,Fuzhou 350013;China);Research Progress of FKBPs[J];Strait Pharmaceutical Journal;2004-03
6 CHANG Yan-ping~1,ZHANG Yan~2,WU Fang~1,ZHANG Ting~2,YANG Wen-xiang~2,ZHANG Chun-gen~2,LIU Da-qun~2(1.College of Life Sciences,Hebei University,Baoding 071002,China;2.College of Plant Protection,Agricultural University of Hebei,Baoding 071051 ,China);Optimization of Fermentation Process for Streptomyces roseoflavus Men-myco-93-63[J];Journal of Hebei University(Natural Science Edition);2006-01
7 WANG Xiao-lei,GAO Hui-ying,JIANG Qin,LIU Gang-san,ZHANG Hua,HE Jian-gong(National Microbial Medicine Engineering & Research Center,New Drug Research and Development Co.,Ltd.of North China Pharmaceutical Corporation, Shijiazhuang 050015,China);Protoplasts Preparation,Regeneration and Mutagenesis of Streptomyces tsukubaensis[J];Journal of Hebei University(Natural Science Edition);2006-01
8 Zhang Xiujun Zhao Yi Wen Shixiang (Department of Agronomy, Hubei Agricultural College, Jingzhou 434103);A Modified Medium for Protoplast Regeneration of Streptomycetes[J];JOURNAL OF HUBEI AGRICULTURAL COLLEGE;1998-04
9 LIU Qiu,YU Ji-cheng,LIU Chang-jian,WANG Yan-ying(Dalian Nationalities University?熏 Dalian 116600,China);Study on protoplast preparation condition of streptomyces chromogenes MY02[J];Hubei Agricultural Sciences;2004-03
10 Zhang Xiujun 1,2) Zhou Qi 1) ( 1) Huazhong Agricultural University, Wuhan 430070; 2) Hubei Agricultural College, Jingsha 434103);STRAIN IMPROVEMENT OF ANITBIOTIC 5102 PRODUCING Streptomyces hygroscopicus var. yingchengensis BY PROTOPLAST FUSION BETWEEN HOMOGENOUS STRAINS Ⅰ. STUDY ON PROTOPLASTS FORMATION, REGENERATION AND FUSION[J];JOURNAL HUAZHONG(CENTRAL CHINA) AGRICULTURAL UNIVERSITY;1997-01
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 WANG Fu zhuan, LIANG Qiu xia, LI Zong wei, WANG Yan ping, WU Jian (Henan Provincial Key Lab of Ion Beam Bioengineering Lab, Zhengzhou University, Zhengzhou, 450052);Applications of Mutation and Screening in Microbiology Breeding[J];Journal of Luoyang Teachers College;2002-02
2 MU Yun-long (New Drug Research and Development Center in North China Pharmaceutical Group,Shijiazhuang 050015,China);Progress of the Research on Rapamycin[J];Hebei Chemical Engineering and Industry;2010-01
3 Zhang Li and Jia Xiao-jing(New Drug Research and Development Center,North China Pharmaceulical Co.,Shijiazhuang 050015);Screening of mutant overproducing sirolimus with gentamicin resistance method[J];Chinese Journal of Antibiotics;2008-01
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 Zheng Youxia Zhao Renjun Xu Xiaoxue Zhang Yifen Zhang Tinglan (Shanghai Institute of Plant Physiology, Academia Sinica);GENETIC STUDIES FOR PLASMID INVOLVING IN QINGFENG- MYCIN BIOSYNTHESIS IN STREPTOMYCES QINGFENGMYCETICUS[J];Acta Genetica Sinica;1980-02
2 Wang Hongzhou Zheng Youxia (Department of Microbiology, Shanghai Institute of Plant Physiology, Aoademia Sinica);Studies on the Formation, Regeneration of Protoplasts and Recombination by Protoplast Fusion in Streptomyces qingfengmyceticus[J];Acta Genetica Sinica;1982-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved