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《Journal of Laparoscopic Surgery》 2009-10
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The biological behavioral influence of artificial pneumoperitoneum on colon carcinoma cell

TIAN Yong-gang1,XU Jun2,LIU Chang2.1.The 2nd Affiliated Hospital of Harbin Medical University,Harbin 150086,China;2.The 4th Affidiated Hospital of Harbin Medical University  
Objective:To discuss the safety and feasibility of artificial pneumoperitoneum in the colon carcinoma operation and observe the influence of different pneumoperitoneal medium and PD98059 in the growth condition of colon carcinoma LS-174T cell in vitro.Methods:(1)experimentation grouping:control group,drug control group,CO2 group,drug CO2 group,He group,drug He group(ultimate PD98059 density in all drug group was 100μmol/L).(2)measurement of the cell proliferation condition by MTT.(3)detection of cell apoptosis ratio by AO/EB double fluorescein stain.(4)test of single cell proliferative capacity by soft agar clone formation experiment.(5)examination of the expression of HIF-1α and VEGF in colon carcinoma cell.Results:The growth speed and proliferating capacity of colon carcinoma cell LS-174T after CO2 pneumoperitoneum effect were higher than that of control group in vitro,while He group was significantly lower than the control group.Cell apoptosis ratio in He group was higher than that in CO2 group and control group.Positive expression of HIF-1α and VEGF in CO2 and He artificial pneumoperitoneum was higher than control group,and the expression in CO2 group was higher than He group,with both the significant difference.The cell growth speed,proliferation capacity,positive expression of HIF-1α and VEGF in PD drug group was lower than handled group with significant difference.Conclusions:CO2 pneumoperitoneum can promote the growth of colon carcinoma cell LS-174T,while He pneumoperitoneum and PD98059 can inhibit its growth relatively.
【Fund】: 黑龙江省教育厅课题资金资助(编号:11511196)
【CateGory Index】: R735.35
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