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《Radiation Protection》 2006-06
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STUDY ON RELATIONSHIPS OF TIME-RESPONSE AND DOSE-RESPONSE OF RADIATION-INDUCED DNA DAMAGES USING SINGLE CELL GEL ELECTROPHORESIS FOR RAPID DOSE ASSESSMENT

Jiang Yaqi~(1,2) Chen Ying~2 Liu Fenju~1 Li You~(2,3)(1.School of Radiology Medicine and Public Health,Soochow University,Suzhou,215123;2.Beijing Institute of Radiation Medicine,Beijing,100850;3.School of Public Health,Nanhua University,Hunan hengyang,421002)  
The relationships of time-response and dose-response of DNA damages of peripheral blood cell induced by()~(60)Co γ radiation were studied using single cell gel electrophoresis and the feasibility of SCGE as a biological dosimeter was discussed.Mice and human peripheral blood cells were irradiated in vivo and in vitro respectively,for drawing up curves of time-response and dose-response.In vivo,DNA damages in mice peripheral blood cells were the most serious immediately after irradiation,and were essentially repaired 2 h after irradiation and had no statistically significant difference(p0.05) between irradiation and control groups 24 h after irradiation.In vitro,TM value of human peripheral blood cells increased in a dose dependent manner,and a extremely statistically significant difference was observed between irradiation and control groups after irradiation immediately.The dose-response relationship well fitted with linear model: Y=0.3619+2.1834D(r =0.9946).However,there has been no statistical difference(p0.05)between irradiation and control groups 6 h after irradiation.The results indicate that SCGE is a rapid,sensitive method for examining radiation-induced DNA damages,and has showed a well linear dose-response relation although it can only measure in a limited time range as damages repaired more rapidly.
【CateGory Index】: R144
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