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《Radiation Protection》 2007-03
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STUDY OF GENOMIC INSTABILITY OF CHL CELLS INDUCED BY DIFFERENT DOSE OF γ RAY

Zuo Yahui Guo Xianhua Wang Xiaoli Wang Zhongwen(China Institute for Radiation Protection,Taiyuan,030006)Geng Xiaohua(Shanxi University,Taiyuan,030006)  
In this paper,the direct effect of lung fibroblasts(CHL) cell in China hamster and genomic instability induced by 60Co γ rays was studied by the methods of single cell gel electrophoresis(SCGE) and Cytokinesis-block micronucleus assay.The fast-growing cells were grouped and then irradiated by different doses of gamma ray.Parts of surviving cells were studied by the methods of single cell gel electrophoresis(SCGE) and micronucleus frequency(MF).At the same time,the others were continuously cultured.After 33 passages of subculture,the progenies were secondly irradiated with 2 Gy gamma rays.And then SCGE and MF were performed.The results showed that the second damage was correlated with the original irradiation dose.It could be concluded that the gamma rays not only induce the immediate biological effects in CHL cells,but also can lead to the genomic instability in the descendants.
【Fund】: 中核基金资助项目(项目号:416050202)
【CateGory Index】: R144
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