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APPLICATION OF SINGLE CELL GEL ELECTROPHORESIS ASSAY IN RADIATION BIODOSE ESTIMATION AND ENVIRONMENT MONITORING

Yan Xuekun Chen Ying Du Jie Zhang Xueqing(Institute of Radiation Medicine,Academy of Military Medical Sciences,Beijing,100850)  
Single cell gel electrophoresis(SCGE) or comet assay(CA) is a rapid,simple,sensitive,reliable and inexpensive method for detecting DNA damage in eukaryotic individual cells.The methodology and principles of SCGE are first introduced,such as analytical parameters,automatic analysis of images,advantages of SCGE etc.,and then its application is investigated and discussed,including radiation protection and environment monitoring,organism monitoring,biological dose estimation.
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