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《Journal of Nurses Training》 2011-17
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Investigation on bacterial origin of sterile packages for war readiness in Zhangzhou

Zhang Ying,Wang Xianyuan,Zhou Juan(Chinese Armed Police Force Hospital of Beijing,Beijing,100027)  
Objective To investigate the origin of bacteria of sterile packages for war readiness under natural environment in Zhangzhou.Method Genomic DNAs of Bacillus cereus,Staphylococcus hominis,Micrococcus luteus,Staphylococcus warneri in sterile packages from war readiness and from air bacteria strains in war readiness warehouse were extracted,and then used as templates to amplify the 16 SrRNA and 23 SrRNA internal transcribed spacer(ITS)sequences by PCR.The PCR products were extracted and sequenced,and the sequences homology analysis was performed with BLAST.Result Amplification products of DNA PCR from Bacillus cereus,Staphylococcus hominis,Micrococcus luteus,Staphylococcus warneri were about 850 bp,1 000 bp,1 100 bp,1 000 bp respectively;The 16 SrRNA-23 SrRNA ITS sequences of bacteria strains in sterile packages shared 98% to 99% homology with those from the air bacteria strains in war readiness warehouse.Conclusion The bacteria in sterile packages for war readiness come from the air in war readiness warehouse under natural environment in Zhangzhou.
【Fund】: 全军医学科学技术研究“十一五”计划专项课题项目(编号:06Z078)
【CateGory Index】: R82
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