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《Journal of Nurses Training》 2011-22
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Experimental study on expiration time of readiness sterile packages in different regions

Zhang Ying,Wang Xianyuan,Zhou Juan(The General Hospital of Beijing Armed Police Force,Beijing,100027)  
Objective To study the expiration time and origin of bacteria for the readiness sterile packages reserve in inland littoral and highland region under natural environment.Method Prepared operation dressing package,apparatus package as well as dressing and apparatus mixed package with double-cotton was deposited in readiness storage boxes under natural environment after pulsing vacuum steam sterilization.Three of every kind of packages were put in a storage boxes and taken for bacterial culture at different time-points.Meanwhile,the bacteria strains in combat readiness warehouse' air and in the storage boxes were cultured and performed with homology analysis.Result Expiration time of readiness sterile packages is 24 d in Zhangzhou region,40 d in Chongqing region and 91 d in Lhasa region.In three regions,the major bacteria were G+ bacilli and G+ staphylococc in readiness sterile packages,combat readiness warehouse 'air and storage boxes.In Zhangzhou,Chongqing and Lhasa,the 16SrRNA-23SrRNA ITS sequences of bacteria strains in sterile packages shared about 98%~99%,97%~100% and 99%~100% homology with which between the air bacteria strains in combat readiness warehouse and the storage boxes respectively.Conclusion The storage environment is the most influential role on expiration time of readiness sterile packages.The bacteria in sterile packages of strategic reserve came from the air of combat readiness warehouse.
【Fund】: 全军医学科学技术研究“十一五”计划专项课题项目(编号:06Z078)
【CateGory Index】: R472
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