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《Journal of Radioimmunology》 2005-02
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Detection of Serum Anti-Sperm Antibody in Infertile Couples with Dot-Immunogold Filtraton Assay (DIGFA)

Xie Xiaoxian Changshu No. 5 People’s Hospital, Changshu, Jiangsu (215500)  
Objective To develop a new method for rapid detection of serum anti-sperm antibody in infertile couples. Methods Human sperm antigen was prepared from pooled semen specimens of fertile males. Nitro-cellulose membrane was used as solid-phase carrier of the antigen. Colloidal gold pellet combined goat anti-human IgG was taken as labelled antibody. A dot-immunogold filtration assay system was established for test of serum anti-human sperm antibody. Serum specimens from 137 infertile couples were tested and the result compared with flat from ELISA. Results The human sperm antigen would react with the anti-sperm antibody in the tested serum over the cellulose membrane through filtration and the result could be read with naked eye within 6 minutes. In this study of 137 infertile coupled, the anti-sperm antibody was positver in 21.9% of the female serum specimens and 13.19% of the males. Compared with the result from ELISA, the consistency rate was 96.1%. The sensitivity of the assay was 90.2% and specificity was 95.4%. The preparation was stable after 6 months refrigerator storage. Conclusion This newly developed DIGFA is very adequate for rapid detection of anti-sperm antibody and deserves popularization.
【CateGory Index】: R446.6
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