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《Chinese Journal of Forensic Medicine》 2006-01
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Y-STRs Multiplex Amplification and Validation for Forensic Scieuce

SHI Mei-sen, LI Ying-bi, YU Xiao-jue, et al. / Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041,China  
Objective In order to increase significantly the discriminatory potential of Y-STR systems available to the forensic community , We have developed and validated a four-color fluorescence multiplex PCR system consisting of 7 male-specific and polymorphic Y-STRs. Methods We designed three sets of constant primers chimerited into three groups of 7 Y-STR's specific primers and utilized fluorescence- labeled these three sets of constant primers to amplified simultaneously 7 Y-STRs in a reaction tube. Allele and haplotype frequencies at these Y-STRs were screened by ABI PRISM310 Genetic Analyzer and analyzed by Genotyper software. Results Following optimization of the polymerase chain reaction , DYS434, Y-GATA-A10,DYS531, DYS557 , DYS456 , DYS444 and DYS448 in a sample of 120 unrelated males , showed4, 5, 5, 8, 8, 6,7 alleles respectively. A total of 101 different haplotypes was identified,of which 89 (88. 11% ) were found in single individuals. The overall haplotypes diversity reached 0.9958. To the one case of mixture stains, our multiplex system drawn conforming conclusion comparing to the result of Y-STR genotypes in suspect's blood sample. Conclusion Our results show that the multiplex system of 7 Y-STR will be very powerful for establishing Y-STR database, the paternity testing and mixture stains identifying.
【Fund】: 国家自然科学基金资助项目(30271446);; 高校博士点专项科研基金资助项目(20020610044)
【CateGory Index】: D919
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【Co-citations】
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1 SONG Xing-bo,FAN Hong,YING Bin-wu,LU Xiao-jun,WANG Jun,YE Yuan-xin Department of Laboratory Medicine,West China Hospital,Sichuan University,Chengdu 610041,China;Analysis of the genetic polymorphism of 17 Y-chromosomal short tandem repeat loci in the Han population in Chengdu[J];Journal of Southern Medical University;2009-10
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