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《Chinese Journal of Forensic Medicine》 2006-02
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Preparation of rapid test strip of colloidal gold labeled anti-ketamine monoclonal antibody for the detection of ketamine

(~△ZENG Li-bo,CHEN Lian-kang,HU Xiao-long,et al.~△ Shanghai Forensic Science and Technology Research Institute,Shanghai 200083,China)  
Objective To develop an easy to use and accurate colloidal gold immunochromatographic test for detecting ketamine.Methods Prepare a complete antigen by conjugating small molecule ketamine to KLH protein.The monoclonal antibody against ketamine was generated by the standard protocol.Colloidal gold was prepared by reduction of HAuCl4 with trisodium citric acid and labeled with anti-ketamine monoclonal antibody.The complete antigen Ketamine-BSA was coated on the nitrocellulose membrane on the test strip.Different intensities of the purple line on the strip could be seen by naked eyes as a result of the competition for labeled antibody binding sites between solid phase ketamine-BSA and free ketamine which may be present in the samples.Results Five hundred urine samples were tested for ketamine by immunochromatographic and GC/MS methods.The cut-off value was established to be 1000ng/ml,the accuracy was 97.6%.Conclusion The reported immunochromatographic test is a sensitive,specific,rapid and easy to use assay for detecting ketamine in the samples.
【Fund】: 上海市科委重点科研项目基金(03251207)
【CateGory Index】: D919
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