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《Chinese Journal of Forensic Medicine》 2009-01
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A study on genetic polymorphisms of 11 canine STR loci in tibetan mastiff

(JIN Xin1,3,TU Zheng2,JIANG Chengtao2,ZHAO Xingchun2,YE Jian2/1.Chinese People′s Public Security Uninversity,Beijing 100038,China;2.Institute of Forensic Science of Ministry of Public Security,Beijing 100038,China;3.Hainan Provincial Public Security Department,Haikou 570203,China)  
Objective To develop a multiplex PCR system of canine STR for individual identification and paterntity testing of dogs.Methods 107 tibetan mastiffs were genotyped by using the self-constructed multiplex PCR amplification system containing 11 canine STR loci with ABI 3130 genetic analyzer.Results In addition to one locus less than 60%,the heterozygosity of other 10 loci was higher than 70%,and the polymorphism information content(PIC) of all of 11 loci was more than 0.6.The discriminating power(DP) of these 11 loci was more than 0.826,in which nine loci surpassed 0.938.The probability of exclusion(EP) for each locus ranged from 0.401 to 0.749 except TETRA locus which the EP was 0.267.The match probability for these 11 loci was estimated to be 1.08×10-13.Conclusion These 11 dog-STR loci are highly polymorphic and suitable for individual identification and paternity testing.
【CateGory Index】: D919
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【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 LIU Qing-shen, FENG Ding-yuan, ZHANG Hui-ming, LIU Shen-fu (College of Animal Science, South China Agricultural University, Guangzhou 510642, China);Genetic Diversity of Chinese Shar-pei Dog Using Microsatellite DNA Markers[J];Zoological Research;2008-01
2 DU Wei-an1, XIONG Yong-hua1, YE Jun-hua2, YANG Qian-yong2 (1. Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China; 2. Police Dog Base in Nanchang, Ministry of Public Security, Nanchang 330100, China);Genetic Polymorphism of Eleven Canine STR Loci[J];Journal of Forensic Medicine;2008-06
【Co-references】
Chinese Journal Full-text Database 5 Hits
1 LIU Qing-shen, FENG Ding-yuan, ZHANG Hui-ming, LIU Shen-fu (College of Animal Science, South China Agricultural University, Guangzhou 510642, China);Genetic Diversity of Chinese Shar-pei Dog Using Microsatellite DNA Markers[J];Zoological Research;2008-01
2 LI Cai-xia 1,LING Feng-Jun 2,TU Zheng 3,ZHENG Xiu-Fen 3 (1.China People's Public Security University,Beijing,100038,China;2.Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing,100101,China;3.Institute of Forensic Science,Beijing,100038,Chin;Genetic Polymorphism of Three STR Gene Loci of the Dog[J];Progress In Veterinary Medicine;2002-05
3 MA Wei1,YE Jun-hua2,MA Chang-shu2,YANG Li-guo3(1.Institute of Sci-tech Information,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China; 2.Nanchang Police Dog Base,Nanchang 330100,China; 3.College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,China);Analysis on genetic diversity of seven police dog breeds by AFLP[J];Guangdong Agricultural Sciences;2007-07
4 DU Wei-an1, XIONG Yong-hua1, YE Jun-hua2, YANG Qian-yong2 (1. Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China; 2. Police Dog Base in Nanchang, Ministry of Public Security, Nanchang 330100, China);Genetic Polymorphism of Eleven Canine STR Loci[J];Journal of Forensic Medicine;2008-06
5 YE Jun-hua~1,MA Jun-wu~1,YANG Qian-yong~1,MA Chang-shu~2,GUO Yuan-mei~2, XU Li~1, HUANG Lu-sheng~2 (1. Nanchang Police Dog Base of the Ministry of Public Security, Nanchang 330100,China; 2. The Key Open Laboratory of Animal Biotechnology of Jiangxi Province,JAU, Nanchang 330045,China);Identification of Police Dog Parentage Based on Ten STR-DNA Polymorphisms[J];Acta Agriculturae Universitis Jiangxiensis;2005-01
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 YE Jun-hua~1,MA Jun-wu~1,YANG Qian-yong~1,MA Chang-shu~2,GUO Yuan-mei~2, XU Li~1, HUANG Lu-sheng~2 (1. Nanchang Police Dog Base of the Ministry of Public Security, Nanchang 330100,China; 2. The Key Open Laboratory of Animal Biotechnology of Jiangxi Province,JAU, Nanchang 330045,China);Identification of Police Dog Parentage Based on Ten STR-DNA Polymorphisms[J];Acta Agriculturae Universitis Jiangxiensis;2005-01
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