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《Chinese Journal of Forensic Medicine》 2012-01
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Development of six Y-STR fluorescent-multiplex amplification system and its application

(Mei Kun1,Xu Nianlai2,Yi Shaohua1,Yang Rongzhi1,Huang Daixin1/1.Department of Forensic Medicine,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China;2.Xiaoshan Sub-bureau,Hangzhou Public Security Bureau,Hangzhou 311200,China)  
Objective To develop a fluorescent-multiplex amplification system for six Y-STR loci and evaluate its application value in forensic medicine.Methods Multiplex amplification primers of six Y-STR loci(DYS444,GATA-A7.2,GATA-A10,DYS390,GATA-A7.1 and DYS443) were designed.The total volume of amplification system was 20μL,including 0.5~10ng of DNA template.Electrophoresis of PCR production was carried out on 3130 genetic analyzer.The result was analyzed with Genemapper ID v 3.2 software.Every allele was named according to the allelic ladder.The properties of the amplification system were evaluated,including its specificity,accuracy,equilibrium,sensitivity of amplification and analysis ability for mixed samples.Results The best amplification result could be attained from 0.5~10ng DNA template or in the condition of 200μmol/L of dNTP and 1.5mmol/L of Mg2+ in amplification system.The alleles of six Y-STR loci were successfully tested,showing unambiguous genotyping,amplification equilibrium,strong specificity and good reproducibility.The amplification system could satisfy the need of forensic practice.In study of population genetics for Han population in Hubei province,the gene diversity values(GD) for each locus ranged from 0.580 3 to 0.722 3.158 kinds of haplotypes were identified and the haplotype diversity(HD) was 0.995 8.Conclusion The six Y-STR fluorescence multiplex amplification system is reliable,and can further improve the discrimination power combined with other commercial Y-STR typing kits.
【CateGory Index】: D919
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【Citations】
Chinese Journal Full-text Database 2 Hits
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【Co-citations】
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1 LIU Chao1, 2, CHEN Ling1, CHEN Xiao-hui2, LIU Chang-hui2, WANG Hui-jun1 1Institute of Forensic Medicine, Southern Medical University, Guangzhou 510515, China; 2Guangzhou Criminal Science and Technology Institute, Guangzhou 510030, China;Polymorphism of 12 Y-STR loci in Guangzhou Han population and its application in forensic medicine[J];Journal of Southern Medical University;2007-05
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