Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Analysis and Testing Technology and Instruments》 2005-01
Add to Favorite Get Latest Update

Study on the Analysis of Chlorogenic Acid in Tobacco

CHEN Kai-bo,TIAN Zhen-feng,CHEN Chuang(Technology Center of Wuhu Cigarette Factory, Wuhu 241002, China)  
A simple and rapid method has been developed for determination of chlorogenic acid in tobacco by RP-HPLC and the extracting process was optimized. The quantitative results were obtained by the internal standard method. The results indicated that the optimum extraction process is as follows: the content of ethanol is 60%,the temperature of ultrasound is 50 ℃,the pH of ethanol solution is 4, the time of extraction is 45 minutes. The method has good linearity with correlation coefficient of 0.9999.The limit of detection based on signal-to-noise ratio of 3 was 1 4×10~(-2) mg/10 mL. The recovery of chlorogenic acid in tobacco was 101.5%,with RSD of 1.58%.
【CateGory Index】: TS47
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 3 Hits
1 Zhang Qiufang,Liu Bo,Shi Huai,Huang Sufang Biotechnology Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350003, China;Rapid Determination of Chlorogenic Acid in Flowers of Lonicera japonica[J];Journal of Fujian Medical University;2006-04
2 TONG Bao-yun1 ,MA Wen2 (1.Technology Center of Anhui Branch of China Tobacco Industry Co.,Hefei 230088,China; 2.School of Chemistry and Chemical Engineering,Anhui University,Hefei 230039,China);Study on Ultrasonic Extraction of Chlorogenic Acid in Tobacco[J];Anhui Chemical Industry;2009-05
3 LI Xin,WANG Zheng(College of Bioscience and Biotechnology,HNAU,Changsha 410128,China);Determination of chlorogenic acid constituent in sweet potato leaves by HPLC[J];Journal of Hunan Agricultural University(Natural Sciences);2009-02
Chinese Journal Full-text Database 1 Hits
1 Zone Jiulian Mountain of Jiangxi,Wang Hongwu,Tang Minyan,Sun Lingfeng,Ji Qingsen(Department of Chemistry,Jiangxi Normal University,Nanchang 330027;);Study on the Extraction of Chlorogenic Acids from the Leaves of Eucommia Ulmoides[J];Journal of Jiangxi Normal University (Natural Sciences Edition);1997-04
Chinese Journal Full-text Database 10 Hits
1 Zhong Bo Zhu Lieshu Xie Huiling Qi Shaowu He Peng Lin Yueping(Hunan agriculture university changsha 410128);Effects of Different Top Dressing Frequence on Growth and Chemical Composition of Flue-cured Tobacco[J];Anhui Agricultural Science Bulletin;2008-09
2 YU Jian-jun et al (National Tobacco Cultivation & Physiology & Biochemistry Research Center,Henan Agricultural University,Zhengzhou,Henan 450002);Change of Variety of Nonvolatile Organic Acids and High Fatty Acid of Red Tobacco[J];Journal of Anhui Agricultural Sciences;2006-03
3 LUO Yi et al (National Engineering Research Center for Wheat,Henan Agricultural University,Zhengzhou,Henan 450002);Effect of Malic Acid on the Flue-cured Tobacco Quality[J];Journal of Anhui Agricultural Sciences;2006-19
4 LI Guo-xi et al(College of Animal Husbandry and Veterinary Medicine,Henan Agricultural Univercity,Zhengzhou,Henan 450002);Effect of Flos lonicerae on Chicken Colibacillosis[J];Journal of Anhui Agricultural Sciences;2006-20
5 LOU Yi et al(Henan Agricultural University,Zhengzhou,Henan 450002);Effect of the Organic Acids on Growth-development of Flue-cured Tobacco[J];Journal of Anhui Agricultural Sciences;2006-24
6 WEI Jian-yu et al(College of Agronomy,Guangxi University,Nanning,Guangxi 530005);Experiment on the Adaptability of Flue-cured Tobacco Varieties[J];Journal of Anhui Agricultural Sciences;2008-06
7 ZHANG Ai-min et al (Department of Biology,Jining College,Jining,Shandong 273200);Study on the Chlorogenic Acid Content and Browning Degree of Flue-cured Tobacco under Different Aging Conditions[J];Journal of Anhui Agricultural Sciences;2008-10
8 ZHANG Feng-xia et al(College of Agronomy,Henan Agricultural University,Zhengzhou,Henan 450002);Effect of Cow Feces Application on the Flue-cured Tobacco Yield and Quality[J];Journal of Anhui Agricultural Sciences;2008-33
9 ZHANG Jian-hai et al(College of Agronomy and Biotechnology,Southwest University,Chongqing 400716);Study on Optimum Extraction Process for Chlorogenic Acid from Lonicera macranthoides Hand.-Mazz.of Xiushan[J];Journal of Anhui Agricultural Sciences;2008-35
10 DONG Xiong-zi et al (Department of Chemistry and Chemical Engineering,Hefei Normal College,Hefei,Anhui 230061);Extraction of Chlorogenic Acid from Different Plants[J];Journal of Anhui Agricultural Sciences;2009-01
Chinese Journal Full-text Database 10 Hits
1 Li Yuezhong Zong Wei Dong Haili(Departmen of Chemistry Engineering,Huainan Union University,Huainan 232001,China);Study on Ultrasonic Extraction of Chlorogenic Acid from Chrysanthemums[J];Anhui Agricultural Science Bulletin;2008-09
2 GU Xun-gang~1,TONG Jun-hua~2(1.School of Resources and Environmental Science,Anhui Agricultural University,Hefei 230036;2.Bureau of Quality and Technical Supervision of Chaohu City,Chaohu 238000);Simultaneous determination of six polyphenols in tobaccos by high performance liquid chromatographic method[J];Journal of Anhui Agricultural University;2006-01
3 Zone Jiulian Mountain of Jiangxi,Wang Hongwu,Tang Minyan,Sun Lingfeng,Ji Qingsen(Department of Chemistry,Jiangxi Normal University,Nanchang 330027;);Study on the Extraction of Chlorogenic Acids from the Leaves of Eucommia Ulmoides[J];Journal of Jiangxi Normal University (Natural Sciences Edition);1997-04
4 ZHANG Shou-yao, ZHOU Ben-jie, WANG YanDepartment of Pharmaceutics, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China;Determination of L-sesamin and L-asarinin in Zanthoxylum(Roxb.) DC. by high performance liquid chromatography[J];Journal of First Military Medical University;2002-07
5 Peng Ruixing (Municipal Environmental Monitoring Station of Fujian, Fujian, Sanming, 365000);Determination of k~+、Na~+、Ca~(2+) and Mg~(2+) in rainfall by FAAS[J];Fujian Analysis&Testirg;2004-01
6 ZHANG Qiu-fang~1, LIU Yi-ping~2, LIU Bo~1, XIE Xiao-dan~2, LIN Pei-zhang~2(1. Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China; 2. Fujian Tobacco Industry Company, Xiamen, Fujian 361004, China);Research progress in tobacco polyphenol substance[J];Fujian Journal of Agricultural Sciences;2006-02
7 ZHANG Ping-quan,DU Xiu-min,ZHANG Yuan(Technology Center of Huaiyin Cigarette Factory,China Tobacco Jiangsu Industrial Corporation,Huaian 223002,China);Study on Determinating Polyphenols in Cigarette by Near Infrared Reflectance Spectroscopy[J];Analysis and Testing Technology and Instruments;2007-03
8 Zhang Jianxun, Li Kean * (Department of Chemistry, Peking University, Beijing 100871) Liu Huifang, Chen Qiufang (Xinzheng Cigarette Factory, Xinzheng 451150);The Analysis of Components in Cigarette Using Pyrolysis Gas Chromatography-Mass Spectrometry[J];Chinese Journal of Analytical Chemistry;2004-05
9 Zhang Tian1, Dong Xuechang~*1 , Wu Fangping1,Yang Guangyu2,Qiao Yongfeng1 ~ 1(School of Chemistry and Biology Technology, Yunnan University for Nationalities,Kunming 650031) ~ 2(Yunnan Academy of Tobacco Science, Kunming 650106);Study on Solid Phase Extraction and High Performance Liquid Chromatography Method for the Determination of Ten Polyphenols in Tobacco[J];Chinese Journal of Analytical Chemistry;2005-03
10 Li Shuguang 1, Zhao Jingmei 1, Wang Wenxia 1, Jin Yu 1,2 , Du Yuguang *1 1(Danlian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023) 2(Liaoning Normal University, Dalian 116029);Simultaneous Determination of Ten Sulfa Drugs in Chicken Meatby High Performance Liquid Chromatography- Coulometric Array Detector[J];Chinese Journal of Analytical Chemistry;2005-04
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 XU Zeng-han1,WANG Neng-ru1,LIU Ling1,ZHOU Hui-ling1,ZHANG Ying2,PENG Hui-jun2,JING Jun-ling2(1.Research Center of Tobacco and Health,University of Science Technology of China,Hefei 230051,China; 2.Sanming Branch of Fujian Tobacco Company,Sanming 365000,Fujian,China);Effects of Curing Technique and Maturity and Sampling Ways on Polyphones of Tobacco Leaves[J];Hubei Agricultural Sciences;2007-04
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Yan Xinfu (Henan Academy of Agricultural Sciences, Zhengzhou )Han Jinfeng (Henan Agricultural University ,Zhengzhou);RESEARCH ADVANCE IN POLYPHENOLIC COMPOUNDS OF TOBACCO[J];Acta Agriculturae Boreali—Sinica;1987-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved