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《Analysis and Testing Technology and Instruments》 2010-01
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Pretreatment of Sample Based on Macroporous Adsorptive Resin and Content Determination of Swainsonine in Oxytropis Kansuensis Bunge by HPLC

DUAN Qiu-yan1,2,HUANG Xin-yi1,Li Chen1,NI Jing-man2,DI Duo-long1,2 (1.Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province,Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences,Lanzhou 730000,China;2.Pharmaceutical School,Lanzhou University,Lanzhou 730000,China)  
In order to eliminate the effect of impurity on the content determination of swainsonine in Oxytropis kansuensis Bunge,macroporous adsorptive resin(MAR) has been used as a sample pretreatment technology.The sample purified with MAR was performed by HPLC and the swainsonine content in Oxytropis kansuensis Bunge was determined.The spiked recovery ratio was 92.81% with a RSD of 3.67%.RSDs of intra-and inter-day precisions of the method were 1.40% and 3.98%,respectively.The linear range was 0.022~0.730 mg/mL and the detection limit was 0.010 mg/mL.The result showed that the proposed method can remove impurity effectively and is suitable for the content determination of swainsonine in Oxytropis kansuensis Bunge.
【Fund】: 中科院知识创新工程重要方向项目(No.KGCX2-SW-213-08);; 中科院“百人计划”项目
【CateGory Index】: R284
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