SINGLE SWEEP OSCILLOPOLAROGRAPHIC AND NEOPOLAROGRAPHIC DETERMINATION OF VITAMIN K_3
Yuan Zhuobin Zhang Jun (Graduate School,University of Science and Technology of China,Academia Sinica,Beijing)
Vitamin K_3can be detected or determined either by the single sweep oscillopolarographic method or by the neopolarographic method (convolution voltammetry). The extraction procedure was not necessary in clinical trail. Using a base solution of 0.1mol/L NaClO_4 -0.01mol/L HClO_4, there is a linear relation between the peak height (i_p" or e_p") and concentration within the range 1×10~(-8)—5×10~(-5) mol/L (3.51ng/ml—17.5μg/ml). As a result, this linearity can be used for quantitative analysis. At 25℃ the respective peak potentials and the detection limits are -0.78V (SCE), 5×10~(-9) mol/L (1.75ng/ml), and -0.76V (Ag/ AgCl), 4×10~(-9) mol/L (1.40ng/ml) for two methods. The samples of injection vitamin K_3 and plasma vitamin K_3 were determined directly by standard addition method and calibration curve method. All experiments were finished in an hour. Besides, the electrode process is reversible in two steps of single electron reduction controlled by diffusion.