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《Chinese Journal of Analytical Chemistry》 1989-07
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Fang Bin Hu Shengshui Li Peibiao Zhao Zaofan (Department of Chemistry,Wuhan University)  
Single sweep polarography has been used to study the reduction of kanamycin. A sensitive derivative cathodic polarographic wave appears in a medium of 0.01mol/L borate 0.01 mol/L sodium hydroxide and 0.03% formadehyde. The peak potential is -1.3V (vs SCE). It can be used for the determination of kanamycin over a range of 2.0×10~(-7) mol/L to 3.0×10~(-5)mol/L. The detection limit is 1.98×10~(-7)mol/L. Satisfactory results have been obtained in the determination of trace kanamycin in drugs. The recovery of pure kanamycin is 98.0%. Polarographic measurements are performed rapidly and conveniently. The interference of drugs and ions, the behaviour of polarographic wave and the mechanism of electrode reactions have been investigated. The number of electron-transfer and reactive H~+ in the reaction have also been determined.
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