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A New Development Luminescence Label Synthesis and Properties of 6-Coumarinsulphonyl Chloride

Yang Shizhong, Li Longdi~* (Department of Chemistry, Tsinghua University, Beijing 100084)  
A new development luminescence label, 6-coumarinsulphonyl chloride(6-CSC1), has been synthesized, and checked by thermoanalysis, IR and MS-GC technique etc.. Effects of pH, lighting and surfactant on the fluorescence properties of its aqueous solution, and the reactiveness to amino acids etc. are studied. The restults show that both basic condition and enough lighting are necessary conditions for obtaining stabilized and strong fluorescence from 6-CSC1 aqueous solution, and quaternary ammonium salt surfactant can enhance its fluorescence intensity. It also showed that this simple derivative of coumarin can be used as a luminescence labeling reagent for amino acid, amines etc. which has reactivity with SO_2Cl group.
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