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《Chinese Journal of Analytieal Chemistry》 1998-04
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Application of Fluorescence Resonance Energy Transfer Technique in Bioanalysis

Wei Yinan, Li Yuanzong, Chang Wenbao, Ci Yunxiang (College of Chemistry and Molecular Engineering, Peking University, Beijing l0087l)  
Application of fluorescence resonance energy transfer (FRET) technique in bio- analysis are reviewed with 53 references. The review begins with a summary of Forster' S theory, and some of the most commonly used experimental strategy, including the selection of donor-acceptor couple, labeling methods and the detection of energy transfer efficiency. The application of FRET in the study of the structure and function of biological macro- molecules, in immunoassay and in DNA hybridization assay are discussed in subsequent sec- tions. Finally, the prospects for the future application of this technique is conducted.
【CateGory Index】: O657.3
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