Electrochemical Behaviors of Dioxyribonucleic Acid at Mercury Film Electrode
Wu Jintian, Zhou Jianzhang, Huang Yin, Luo Jin, Lin Zhoghua (State Key Laboratory for Physical Chemistry of Solid Surfaces, Department of Chemistry, Institute of Physical Chemistry, Xiamen University, Xiamen361005)
DNA was studied by means of cyclic voltammetry (CV) with a mercury film electrode (MFE) using conventional CV, differential pulse voltammetry and alternating current voltammetry. The MFE is sufficiently stable and can be used to study electrochemical behaviors of DNA in the negative potential region. This means that MFE is ready to be one kind of solid electrode at which mere useful electrochemical techniques can be carried out, such as spectroelectrochemical techniques. Redos character of DNA treated by pure HClO4 was studied at MFE. It seems that pure HCIO4 would not only bring about the denaturation of DNA but the degradation of it. Pure HCIO4 is not suitable for performing the denaturation of DNA.