Simultaneous Determination of o-,m-,p-Dihydroxybenzene Isomer by Thin-Layer Chromatography
Huang Shaopeng, Xu Jinrui , Wang Qiong (Department of Applied Chemistry, Huaqiao University, Quanzhou 362011)
The simultaneous determination of o-, m- , p- dihydroxybenzene isomers on silica gel plate by thin-layer chromatography were studied. The R, values are 0.59, 0.53 and 0.48 respectively using a developping agent of benzene: ether: glacial acetic acid = 7:2:1 ( V/ V/ V). The detection limits are 1.07 × 10-4 , 1.64 × 10-4 and 1.90× 10-4 mol/L respectively. The determination was not interfered by usual phenols. This method has been applied to the determination of the actual sample with a 96% - 104% recovery.
【CateGory Index】： O657.7