Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Analytical Chemistry》 1999-06
Add to Favorite Get Latest Update

Detennination of Atrazine in Soil Samples by Enzyme-Linked Immunosorbent Assay

Deng Anping , Yang Hong(Department of Sanitary Techniques1 , Department of Experimental Chemistry , West China University of Medical Sciences, Chengdu 610041)Franek Milan ( Veterinary Research Institute, Hudcova 70,621 32 Brno, Czech Republic)  
A highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) method is described for the determination of atrazine in soil samples. When the range of determination was 0.05~5.0μg/L, the concentration of atrazine at 50% B/Bo and the limit of detection were from 14 standardcalibration curves, in the range of 0. 18~0. 24μg/L and 0. 018~0. 021μg/L, respectively. Thecorrelation coefficient of the linear standard curve was 0.9922. The solvent effect was investigated and the standard curves were found to move upward slighdy with increasing the amount of organic solvent (methanol). When methanol or acetonitrile solution was used as the extract solvent (with 20% or 10% of water), the efficiency of extraction of atrazine from soil samples was, under optimized conditions, almost 100% . When the 1:200 diluted extract was used for EUSA, mean recovery rate was 99.6% , with the solvent and matrix effects being almost completely eliminated. The developed method provides a rapid,sensitive and accurate procedure for the determination of atrazine in soil, especially promising for screening and quantitation of large amount of samples.
【CateGory Index】: X592
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 7 Hits
1 CHEN Benjing,TAO Bo(College of Agriculture,Northeast Agricultural University,Harbin Heilongjiang 150030,PRC);Application of immunoassay on analysis of herbicide residues[J];Journal of Northeast Agricultural University;2006-04
2 ZHOU Ning,MENG Qingjuan,WANG Rongjuan,ZHANG Ying(Resources and Environmental Sciences College, Northeast Agricultural University, Harbin 150030, China);Progress on biodegradation of herbicide atrazine[J];Journal of Northeast Agricultural University;2008-09
3 Yang Hong (West China School of Pharmacy, Sichuan University, Chengdu 610041);Comparative Study of Two Coating Methods for Determination of Biotin Concentrations in Biological Samples by Enzyme-linked Immunosorbent Assay[J];Chinese Journal of Analytieal Chemistry;2002-10
4 SUN Tai-fan~(1,2), YE Fei~1(1. College of Science, Northeast University, Harbin 150030, China;2. Heilongjiang Aug. 1st Farming Reclamation University, Daqing 163319, China);APPLICATION OF ENZYME IMMUNOASSAYS TO THE ANALYSIS OF RESIDUAL PESTICIDES[J];Physical Testing and Chemical Analysis Part B Chemical Analysis;2005-03
5 Lu Kangbo(Northeast Agriculture University,Haerbin 150030);Detection of Pesticide Residuces and Metabolites by Enzyme Immunoassay[J];Pesticide Science and Administration;2000-05
6 Yang Mei1,2,Lin Zhongsheng1,Yao Ziwei1,Ma Yong an1(1. National Marine Environmental Monitoring Center,Dalian 116023;2. School of Life Science and Technology,Dalian Fishery University,Dalian 116023 China);Process of study on the herbicide atrazine[J];Pesticide Science and Administration;2006-11
7 Yang Hong (West China School of Pharmacy, Sichuan University Chengdu 610041,P.R.China);Study on an ELISA Method for Determination of Biotin in Yeast Cultural Media for Gene Transformation[J];China Pharmacist;2002-06
【Citations】
Chinese Journal Full-text Database 1 Hits
1 Deng Anping (Department of Sanitary Techniques, West China University of Medical Sciences, Chengdu 610041) Franek Milan (Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic);Determination of Atrazine in Water Samples by Enzyme -Linked Immunosorbent Assay[J];Chinese Journal of Analytieal Chemistry;1998-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 CHEN Benjing,TAO Bo(College of Agriculture,Northeast Agricultural University,Harbin Heilongjiang 150030,PRC);Application of immunoassay on analysis of herbicide residues[J];Journal of Northeast Agricultural University;2006-04
2 ZHOU Ning,MENG Qingjuan,WANG Rongjuan,ZHANG Ying(Resources and Environmental Sciences College, Northeast Agricultural University, Harbin 150030, China);Progress on biodegradation of herbicide atrazine[J];Journal of Northeast Agricultural University;2008-09
3 WU Ya-fei,DENG An-ping(College of Chemistry,Sichuan University,Chengdu 610064,China);Development of an indirect enzyme-linked immunosorbent assay for the determination of levonorgestrel in water samples[J];Chemical Research and Application;2007-06
4 HE Li1,2,HUO Song-min1,YANG Hong3,ZHANG Shou-yao1,DENG An-ping1(1.College of Chemistry,Sichuan University,Chengdu 610064,China;2.The Biogas Institute of Ministry of Agriculture,Chengdu 610041,China;3.College of West China Pharmacy,Sichuan University,Chengdu 610041,China);Determination of trace pharmaceutical residue indomethacin in water samples by an enzyme-linked immunosorbent assay[J];Chemical Research and Application;2008-08
5 Yang Hong (School of Pharmacy, West China University of Medical Sciences Chengdu 610041);DETERMINATION OF SULPHADIMIDINE RESIDUES IN MILK,SWINE PLASMA AND SWINE URINE BY ELISA[J];WEST CHINA JOURNAL OF PHARMACEUTCAL SCIENCES;1999-04
6 SUN Tai-fan~(1,2), YE Fei~1(1. College of Science, Northeast University, Harbin 150030, China;2. Heilongjiang Aug. 1st Farming Reclamation University, Daqing 163319, China);APPLICATION OF ENZYME IMMUNOASSAYS TO THE ANALYSIS OF RESIDUAL PESTICIDES[J];Physical Testing and Chemical Analysis Part B Chemical Analysis;2005-03
7 Lu Kangbo(Northeast Agriculture University,Haerbin 150030);Detection of Pesticide Residuces and Metabolites by Enzyme Immunoassay[J];Pesticide Science and Administration;2000-05
8 Yang Mei1,2,Lin Zhongsheng1,Yao Ziwei1,Ma Yong an1(1. National Marine Environmental Monitoring Center,Dalian 116023;2. School of Life Science and Technology,Dalian Fishery University,Dalian 116023 China);Process of study on the herbicide atrazine[J];Pesticide Science and Administration;2006-11
9 YANG Mei1,2, LIN Zhong-sheng1, YAO Zi-wei1, MA Yong-an1 (1. National Marine Environmental Monitoring Center, Coastal Ecology Key Laboratory, Liaoning Dalian 116023, China; 2. School of Life Science and Technology, Dalian Fishery University, Liaoning Dalian 116023, China);Determination of Triazine Herbicide in Environmental Water by Gas Chromatography-Tandem Mass Spectrometry[J];Modern Agrochemicals;2007-01
10 WANG Ming-qi,YE Fei(College of Science,Northeast Agricultural University,Harbin 150030,China);Determination of Residues Pesticide in Water Environment[J];Agrochemicals;2007-01
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 WAN Yu Anhui Research Institute of Chemical Industry,Hefei 230041,China;The New Development of Sampling Preparation Technique in Pesticide Residue Analysis[J];Anhui Chemical Industry;2006-02
2 Tong Lihua1 Hou Weiguo(1 School of Civil Engineering and Architecture,Anhui Industiral University,Anhui Maanshan243002,2 State Key Laboratory of Environmental Geochemistry,Institute of Geochemistry,Chinese Academy of Sciences,Guizhou Guiyang 550002);Physiological Function of Mycorrhizal and its Application in Contaminated Soils[J];Anhui Agricultural Science Bulletin;2007-17
3 Huang Dezhi et al (Teaching Affairs Office, Anhui Agricultural University, Hefei 230036);Research Progress of Analysis of Pesticide Residue[J];Journal of Anhui Agricultural Sciences;2002-04
4 WU Ying et al(College of Arts and Sciences,Tarim University,Aler,Xinjiang 843300);Rapid Detection Method of Pesticide Residue in Agricultural Environment[J];Journal of Anhui Agricultural Sciences;2007-17
5 LIU Bing et al(School of Plant Protection,Shenyang Agricultural University,Shenyang,Liaoning 110161);Research Progress in the Technology of ELISA for Pesticide Residues[J];Journal of Anhui Agricultural Sciences;2007-21
6 Li Yuebin Wang Yuping Jiang Hong Yang Zhengbao Cheng Guowang (Hefei Fengle Agricultural Chemical Plant,Hefei 230031);Analysis of Acetochlor and Quizalofop-ethyl by Gas Chromatography[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;1997-04
7 ZHOU Qi xing & SONG Yu fang (Open Laboratory of Terrestrial Ecological Process, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China);TECHNOLOGICAL IMPLICATIONS OF PHYTOREMEDIATION AND ITS APPLICATION IN ENVIRONMENT PROTECTION[J];Journal of Safety and Environment;2001-03
8 FAN Zhi-jin~1 , CHEN Jun-peng~1 , AI Ying-wei~2 , LI Zheng-ming~1 (1 Institute of Elemento Organic Chemistry, State Key Laboratory of Elemento Organic Chemistry, Nankai University, Tianjin 300071, China; 2 College of Life Science, Sichuan University, Chengdu 610065, China);A PRELIMINARY STUDY ON HERBICIDE ACTIVITY OF MONOSULFURON-ESTER AND ITS SAFETY TO MAIZE(ZEA MAYZ L.)[J];Journal of Safety and Environment;2004-01
9 LIU Li-li, CHEN Sha, REN Ren ( College of Environment and Energy Engineering, Beijing University of Technology, Beijing 100022, China );Solid-phase Extraction Pretreatment Technique and Analytic Methods of Organochlorine Pesticides[J];Journal of Beijing Polytechnic University;2003-01
10 WANG Jing,WEI Chao-jun,ZHAO Jian-zhuang*,WANG Wen-juan,WANG Chun-na(Department of Basic Science,Beijing Agricultural College,Beijing 102206,China);Synthesis of Deltamethrin Artificial Antigen and Ultraviolet Determination of Coupling Ratio[J];Journal of Beijing Agricultural College;2006-04
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 CHEN Benjing,TAO Bo(College of Agriculture,Northeast Agricultural University,Harbin Heilongjiang 150030,PRC);Application of immunoassay on analysis of herbicide residues[J];Journal of Northeast Agricultural University;2006-04
2 ZHOU Ning,MENG Qingjuan,WANG Rongjuan,ZHANG Ying(Resources and Environmental Sciences College, Northeast Agricultural University, Harbin 150030, China);Progress on biodegradation of herbicide atrazine[J];Journal of Northeast Agricultural University;2008-09
3 Li Xuru,Zhang Qin,Liu Chunli.Amway(China) Co.Limited Guangzhou 510730;Automated microbiological assay for d-biotin in multi-vitamin tablets[J];Guangzhou Medical Journal;2006-06
4 ZHOU Ning1,2, ZHANG Ying2(1. Department of Bioengineering, Jiangsu Food Scien- ces College, Huai′ an Jiangsu 223003, China; 2. College of Resources and Environmental Sciences, Northeast Agricultural University, Harbin 150030, China);Isolation and its characteristics of atrazine degrading bacterium Z_9 from black soil[J];Journal of Northeast Agricultural University;2009-09
5 Che Xiao-qing,Guo Ming* (Department ofChemistry,ZheJiangForestryUniversity,Linan,Zhejiang311300,China);Recent Progress of Enzyme-linked Immunosorbent Assay Technique for Heavy Metal Residues[J];Fujian Analysis & Testing;2009-04
6 WU Yan-bing,YAN Zhen-min,WANG Jian-hua,HAN Qian,LI Shan(School of Resource and Environment,Henan Institute of Science and Technology,Xinxiang 453003,China);Security research on different maize varieties to the 48% atrazine WP[J];Guangdong Agricultural Sciences;2009-12
7 CUI Wei-wei et al (Faculty of Natural Resource and Information Technology,China University of Petroleum(Beijing) Beijing 102249);Advance in Studies on the Harms and Exposure of Pesticide Residues[J];Journal of Anhui Agricultural Sciences;2010-02
8 LUO Zai-quan1,DUAN Yu-xi1,WANG Yuan-yuan2,YIN Li-na1,CHEN Li-jie1(1.Department of Plant Protection,Shenyang Agricultural University,Shenyang 110161;2.Department of Biological Science and Technology,Shenyang Agricultural University,Shenyang 110161,Liaoning,China);Fermentation Broth of Bacteria SnebYK Induced Anti-atrazine Effect in Soybean[J];Soybean Science;2010-02
9 FAN Shi-qi,CAO Yuan-yin,LUO Qiao-yan,QIU Hong-wei (Institute of Plant Immunology, Shenyang Agricultural University, Shenyang 110161, China);The Preparation of Polyclonal Antibody for Trichlorphon Residue[J];Hubei Agricultural Sciences;2009-04
10 Zhou Ning Wang Rongjuan Meng Qingjuan Zhang Ying(School of Resource & Environment,Northeast Agricultural University,Harbin 150030);Screening and identification of atrazine degrading bacteria and their degradation characteristics from black soil in cold area[J];Chinese Journal of Environmental Engineering;2008-11
China Proceedings of conference Full-text Database 2 Hits
1 YUAN Fang,YANG Ren-bin (Institute of Agro-Environment Protection, Hunan Agricultural University, Changsha 410148,China);The Application of ELISA in Pesticide Residue[A];[C];2005
2 Guo Jun1,Zhao Li-qin1,Li Cui-zhi2,Meng Lan-huan1 1.Food Science and Engineering College of Inner Mongolia Agricultural University,Hohhot,China.010018 2.Quality Control Deptment of Inner Mongolia YiLi Group Co.Ltd.Hohhot,China.010080;Discussion on Possible Sources and Routes of Melamine Contamination in Animal Derived Foods[A];[C];2009
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved