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《Chinese Journal of Analytical Chemistry》 1999-09
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Determination of Human Serum Albumin by Aluminium Tetrasulponated Phthalocyanine

Li Donghui, Yang Huanghao, Zheng Hong, Chen Bin, Chen Qiuying, Xu Jingou(The Research Laboratory of the State Education Commission of China of Analytical Science for Material and Life Chemistry, Department of Chemistry, Xiamen University, Xiamen 361005)  
Based on the fluorescence quenching of aluminium tetrasulphonated phthalocyanine (AlS4Pc), a red-region fluorescent dye, reacted with albumin, a new method was developed for the determination of human serum albumin(HSA). Under the experimental conditions, the linear range ofthis assay was 0. 10-4.5 mg/Land the detection limit was 40μg/L. The method has been applied to theanalysis of human serum samples and the results were in agreement well with those achieved by bromocresol green method, which indicated that the method was not only sensitive but also reliable.
【CateGory Index】: O657.3
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