Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《CHINESE JOURNAL OF ANALYTIEAL CHEMISTRY》 2000-02
Add to Favorite Get Latest Update

Phosphorimetric Analysis of Riboflavin

Huang Ruheng (Institute of pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850)  
Riboflavin(vitamin B2, VB2) possesses strong fluorescence but no phosphorescene. It turns to a phosphor after heating at 100℃ for 30 min in 0.1 mol/L NaoH with λex 390 nm, λem 520 nm, life time is 0.73 s. The influence of 16 chemicals on the phosphorescence of riboflavin was investigated. Among them only VB1, folic acid, and typtophan, I- and Ba2+ at 10 ~ 100 times ligher concentration than riboflavin can affected the phosphorescence of riboflavin. A simple sensitive phosphorimetric method for determination of riboflavin was proposed. By using this method the concentration of riboflavin in human plasma and contents in tabets of vitamin B2 and vitamin B complex were estimated.
【CateGory Index】: O657.3
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 5 Hits
1 Huang Ruheng (Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850);Phosphorimetric Assay of Pyridoxine[J];Chinese Journal of Analytieal Chemistry;2001-09
2 Huang Ruheng(Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850);Phosphorescence Properties of Two Estrogens[J];Chinese Journal of Analytical Chemistry;2003-11
3 LI Wei 1,2 ,CAO Ling xian 3,LI Gai ru 2,and JIN Wei jun 2 1 Environment and resource college,Shanxi University,Taiyuan 030006,China 2 Chemistry and Chemical Engineering College, Shanxi University,Taiyuan 030006,China 3 Taiyuan Central St;Applications of Phosphorimetry in Pharmaceutical Analysis[J];Spectroscopy and Spectral Analysis;2002-03
4 LONG Wen-qing College of Chemistry and Chemical Engineering,Jinggangshan College,Jiangxi Province Key Laboratory of Coordination Chemistry,Ji'an 343009,China;Determination of Trace Vitamin B_2 by Fluorimetry after Aqueous Two-Phase Extraction Based on Acetone and Ethanol[J];Spectroscopy and Spectral Analysis;2007-10
5 XIANG Zhaobao1 DAI Chuanyun2 ZHU Liqing21 College of Bio -Information ,Chongqing University of Post and Telecom,4000652 Key Laboratory of Biomechanics&Tissue Engineering under the State Ministry of Education,College of bioengineering ,Chongqing University,400044;PHYSIOLOGICAL AND BIOCHEMICAL CHARACTERISTIC AND FUNCTION OF RIBOFLAVIN[J];;2004-06
【Citations】
Chinese Journal Full-text Database 7 Hits
1 Shuang Shaomin Feng Kecong Liu Changsong (Department of Chemistry,Shanxi University,Taiyuan,030006);Investigation and Comparison of Four Types of Chinese Substrates for Solid Substrate-Room Temperature Phosphorimetry——Crystalline Cellulose Membrane,Thin Layer Chromatography Silica Gel G Plate,Anion Exchange Cellulose Membrane and Filter Paper[J];Chinese Journal of Analytical Chemistry;1991-04
2 Huang Ruheng (Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850);Studies on the Phosphorescence of Thiamine[J];Chinese Journal of Analytieal Chemistry;1997-12
3 Huang Ruheng (Institute of Pharmacology and Toxicology, Academy of Military Medical Sclences, Beijing 100850);The Reaction Mechanism of Copper in Phosphorescence Analysis of Thiamine[J];Chinese Journal of Analytieal Chemistry;1998-08
4 HuangRuheng(Institute of Pharmacology and Toxi-cology,Academy of Military Medical Sciences,Beijing l00850 );Studies on the Phosphorescence Lifetimeand its Application in the Determination ofTryptophan in Biological Tissues[J];ANALYTICAL LABORATORY;1996-04
5 Huang Ru-heng(Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences);A SIMPLE METHOD FOR DETERMINATION OF PHOSPHORESCENCE LIFE[J];Bulletin of the Academy of Military Medical Sciences;1983-03
6 Huang Ruheng;Xu Xu Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences;AN IMPROVEMENT OF PHOSPHORIMETRIC TECHNIQUE[J];Bulletin of The Academy of Military(Medical Sciences);1986-02
7 RH Huang (Institute of Pharmacology and Taxicology, Academy of Military Medical Sciences , Beijing 100850);PHOSPHORIMETRIC ANALYSIS OF CARBAINDOLINE[J];Acta Pharmaceutica Sinica;1993-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Xia Bangying;Effects of fluoroprobes on the phosphorescence of nucleic acids in Bombyx mori and Antheraea pernyi. Sci.Seric.,[J];Acta Sericologica Sinica;1988-02
2 Huang Ruheng , Ji Qing' e (Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850);A Variation in Phosphorescence Life-time Analytic Method for Determination of Free Tryptophan in Blood[J];Chinese Journal of Analytieal Chemistry;1995-03
3 Huang Ruheng (Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850);Studies on the Phosphorescence of Thiamine[J];Chinese Journal of Analytieal Chemistry;1997-12
4 Huang Ruheng (Institute of Pharmacology and Toxicology, Academy of Military Medical Sclences, Beijing 100850);The Reaction Mechanism of Copper in Phosphorescence Analysis of Thiamine[J];Chinese Journal of Analytieal Chemistry;1998-08
5 Huang Ruheng (Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850);Phosphorescence of Thiamine After Ultraviolet Irradiation[J];Chinese Journal of Analytieal Chemistry;1998-11
6 Huang Ruheng (Institute of Pharmacology and Toxicology, Academy of Military Military Medical, Sciences Beijing 100850);A New Phosphorimetric Method for Analysis of Methotrexate after Ultraviolet Irradiation and its Application[J];CHINESE JOURNAL OF ANALYTIEAL CHEMISTRY;2000-07
7 Huang Ruheng (Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850);A Phosphorimetric Method for Determination of Folic Acid[J];Chinese Journal of Analytieal Chemistry;2001-03
8 Huang Ruheng (Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850);Phosphorimetric Assay of Pyridoxine[J];Chinese Journal of Analytieal Chemistry;2001-09
9 Huang Ruheng(Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850);Phosphorescence Properties of Two Estrogens[J];Chinese Journal of Analytical Chemistry;2003-11
10 Huang Ruheng (Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850 );Low Temperature Phosphorescence Method for the Analysis of Diethylstilbestrol after Photochemical Reaction[J];Chinese Journal of Analytical Chemistry;2004-11
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZHAI Hong-yan,YAN Xiao-hang(Anhui Institute for Drug Control,Hefei,Anhui Province 230051);Determination of paeonol in qiju dihuang pills by capillary gas chromatography[J];Anhui Medical and Pharmaceutical Journal;2008-11
2 KOU Wan-qing,KAI Wei-hua,WANG Rong,FANG Ling,LIU Jing(School of Materia Medica,Anhui College of Traditional Chinese Medicine,Anhui Key Laboratory of Modernized ChineseMaterial Medical,Hefei 230031,China);Determination of paeonol in guizhifuling pills by HPLC[J];Anhui Medical and Pharmaceutical Journal;2009-05
3 DING Yu~(1,2),JIANG Xiao-jun~1 (1.School of Chemical Engineering,Anshan University of Science and Technology,Anshan 114044,China; 2.Hygienic School Affiliated,Anshan Normal College,Anshan 114005,China);Determination of vitamin B1 with indication ion square wave polarography[J];Journal of Anshan University of Science and Technology;2005-Z1
4 Zhang Jianhai,Yuan guanghua Pang quanhai,Hao junhu,Wan shuangxiu(College of Animal Science and Veterinary Medicine,Shanxi Agricultural University,Taigu,030801);EFFECTS OF DIFFERENT RIBOFLAVIN ON BLOOD CELL,IMMUNE ORGANS AND PRODUCTION PERFORMANCE IN BROILER[J];Shandong Poultry;2003-02
5 WANG Dan-li,ZHANG Min-hong,WEN Jie,DU Rong,XIE Peng,ZHENG Shan-shan (Institute of Animal Science,CAAS,Beijing100094,China);EFFECT OF DIETARY LEVELS OF RIBOFLAVIN ON PERFORMANCE OF BROILERS UNDER HEAT STRESS[J];Acta Zoonutrimenta Sinica;2003-04
6 WANG Yan-hui WANG An~* XIE Fu(Institute of Animal Nutrition,Northeast Agricultural University,Harbin 150030,China);Effects of Vitamin Be on Growth Performance, Endocrine and Antioxidant Capacity in Laying Ducklings in Cage[J];Chinese Journal of Animal Nutrition;2009-01
7 LIANG Ying1 JIANG Ning2 HE Wenjuan3 REN Chengcai2 WANG Deli1 ZHENG Wenfeng1 WANG Juntao1(1.College of Arts and Science,Heilongjiang Bayi Agricultural University,Daqing 163319,China;2.College of Animal Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319,China;3.Xilingol Vocational College,Xilinhaote 026000,China);Effects of Astragalus Polysaccharide on Growth Performance and Immune Function of Broilers[J];Chinese Journal of Animal Nutrition;2010-04
8 Zhang Fudi, Cai Biqiong, Yang Yuancai (College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002);Measuring of riboflavin in tablets of vitamin B_2 with a fluorescence spectrometer[J];Fujian Analysis&Testirg;2004-Z1
9 Shuang Shaomin Feng Kecong Liu Changsong (Department of Chemistry,Shanxi University,Taiyuan,030006);Investigation and Comparison of Four Types of Chinese Substrates for Solid Substrate-Room Temperature Phosphorimetry——Crystalline Cellulose Membrane,Thin Layer Chromatography Silica Gel G Plate,Anion Exchange Cellulose Membrane and Filter Paper[J];Chinese Journal of Analytical Chemistry;1991-04
10 Guo Xiangqun~*; Xu Jingou; Wang Jinling; Chen Guozhen(Department of Chemistry, Xiamen University, Xiamen 361005);Photochemical Fluorimetric Method——Ⅶ. Simultaneous Determination of Vitamin B_1 and Vitamin B_6[J];Chinese Journal of Analytical Chemistry;1992-08
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 SUN Shu-ting,MA Hong-min,CHEN Xin,ZHANG Nuo,HAN Yan-yan,ZHANG Li-na,WEI Qin(School of Chemistry & Chemical Engineering,University of Jinan,Jinan 250022,China);Studies and Application of the Pharmaceutical Analysis by Modern analytical Technique[J];Analysis and Testing Technology and Instruments;2007-04
2 LI Min and DONG Hui-ru(College of Science,Beijing University of Chemical Technology,Beijing 100029);Determination of ergosterol in yeast cells by HPLC after aqueous two-phase extraction[J];Chinese Journal of Analysis Laboratory;2009-11
3 Ma Weixing(School of Chemical Engineering,Huaihai Institute of Technology,Lianyungang 222005,China);Discussion on Applications of Homogeneous Phase Solvent Extraction in Separation Analysis[J];Guangdong Chemical Industry;2011-04
4 HUANG Lin1,CHENG Xin1,TU Xiao-rong1,LI Kun-tai1,2 (1.College of Biological Science and Technology,Jiangxi Agricultural University,Nanchang 330045,China 2.Nanchang Key Laboratory of Applied Fermentation Technology Nanchang 330045,China);Primary research on the optimization of riboflavin fermentation by Eremothecium ashbyii[J];Guangdong Agricultural Sciences;2010-06
5 MA Ming-Yang QU Ying-Juan LI Chen-Lu(Department of Chemistry and Chemical Engineering,Xi'an University of Arts and Science,Xi'an 710065,P.R.China);Determination of Vitamin B_2 of Various Complex Samples by Fluorescence Spectrometry[J];Chinese Journal of Spectroscopy Laboratory;2011-03
6 LI Qi-Yun LI Hong(Department of Basic Subject,Weinan Vocational and Technical College,Weinan,Shaanxi 714000,P.R.China);Determination of Vitamin B2 Based on Luminol-Cu(Ⅱ) System by Flow Injection Chemiluminescence[J];Chinese Journal of Spectroscopy Laboratory;2011-04
7 LI Li-Mina,b SHANG Qing-Kunba(Center of Analysis and Testing,Beihua University,Jilin,Jilin 132013,P.R.China)b(Faculty of Chemistry,Northeast Normal University,Changchun 130024,P.R.China);Application of Hydrophilic Organic Solvent/Inorganic Salt Aqueous Two-Phase Extraction Technique in Separation and Purification Biologic Substance[J];Chinese Journal of Spectroscopy Laboratory;2011-06
8 LI Jia-Lin Gao Zong-Hua (Pharmaceutical College of Binzhou Medical University(Yantai Campus),Yaitai,Shandong 264003,P.R.China];Determination and Stability of Vitamin B_2 in Vitamin B_2 Tablets[J];Chinese Journal of Spectroscopy Laboratory;2012-04
9 XIE Xiu-juan,SHAO Xin and ZHANG Zhen-xin*(ChemistryandMaterials Science Institute,Huaibei Normal University,HuaiBei 235000);Determination of trace vitamin B2 by fluorescence quenching after cloud point extraction[J];Chinese Journal of Analysis Laboratory;2013-06
10 PAN Zu-ting,GUAN Hong-liang,YUAN Hua-ping,LI Wei,CHEN Guo〖JZ〗(College of Chemistry and Molecular Sciences,Wuhan University,Wuhan 430072,China);Application of Fluorescence Spectrum in Pharmaceutical Analysis[J];Journal of Jishou University(Natural Science Edition);2005-03
【Secondary Citations】
Chinese Journal Full-text Database 8 Hits
1 Zhang Sushe (Centre Laboratory, Shanxi Academy of Agricultural Science) Liu Changsong and Chang Gongmin (Department of Chemistry, Shanxi University, Taiyuan);INVESTIGATION OF CHINESE FILTER PAPER AS A SUBSTRATE FOR SOLID SURFACE ROOM TEMPERATURE PHOSPHORIMETRY[J];Chinese Journal of Analytical Chemistry;1987-10
2 Huang Ruheng , Ji Qing' e (Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850);A Variation in Phosphorescence Life-time Analytic Method for Determination of Free Tryptophan in Blood[J];Chinese Journal of Analytieal Chemistry;1995-03
3 Huang Ruheng (Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850);Studies on the Phosphorescence of Thiamine[J];Chinese Journal of Analytieal Chemistry;1997-12
4 Huang Ru-heng;Yuan Shu-lan;STUDIES ON THE METABOLISM OF CUI XING NING:Ⅰ.A SPECTROFLUOROMETRIC DETERMINATION OF CUI XING NING[J];Bulletin of the Academy of Military Medical Sciences;1980-03
5 Huang Ru-heng(Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences);A SIMPLE METHOD FOR DETERMINATION OF PHOSPHORESCENCE LIFE[J];Bulletin of the Academy of Military Medical Sciences;1983-03
6 Huang Ruheng;Xu Xu Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences;AN IMPROVEMENT OF PHOSPHORIMETRIC TECHNIQUE[J];Bulletin of The Academy of Military(Medical Sciences);1986-02
7 M Zhao and RH Huang (Institute of Pharmacolagy and Toxicology, Academy of Military Medical Sciences, Beijing 100850);THE BIOTRANSFORMATION OF CUI XING NING IN THE ISOLATED PERFUSED RAT LIVER[J];Acta Pharmaceutica Sinica;1990-11
8 RH Huang (Institute of Pharmacology and Taxicology, Academy of Military Medical Sciences , Beijing 100850);PHOSPHORIMETRIC ANALYSIS OF CARBAINDOLINE[J];Acta Pharmaceutica Sinica;1993-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved