Simultaneous Chiral Separation of Two Pairs of Enantiomers Using Series Column of Molecular Imprinted Phase
Wang Jinfang, Zhou Liangmo,Liu Xueliang,Wang Junde, Shang Zhenhua (Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116012)
Molecular imprinting polymers (MIPs) against N-Cbz-L-Ser and N-Caz-L-Ala utilizing acrylamide plus 2-vinylpyridine as combined basic functional monomers were prepared and evaluated by high-performance liquid chromatography. MIPs showed chiral separation ability to the imprinted enantiomers and those with similar structure. Simultaneous chiral separations of N-Cbz-DL-Ser and N- Cbz-DL-Ala were achieved on a series column by connecting N-Cbz-L-Ser imprinted column and N-Cbz- L-Ala imprinted column.It showed great potential application of molecular imprinted chiral stationary in chiral separation of phamarceuticals and their intermediates.