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《Chinese Journal of Analytieal Chemistry》 2001-01
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The Energy Transfer Fluorescence Method for Determination of Trace Phosphorous with Acridine Orange-Rhodamine 6G

Liu Baosheng , Wang Guihua, Sun Hanwen, Bao Suoyan, Huang Yongzhang (Research Center of Physical and Chemical Analysis, Hebei University, Baoding 071002)  
It was found that at the condition of λ ex / λ em =450/556 nm,in the dodecyl benzene sodium sulfonate solution, the effective energy transfer could occur between AO and R6G. The fluorescence intensity of R6G was diminished by molybdophosphate which was formed by the reaction between orthophosphate and molybdate in hydrochloric acid medium. The detection limit of this method was 5 ng/L. The range of determination for phosphorous was 0.05~0.70 μg/L. The relative standard deviation for the determination of 0.5 μg/L phosphorous was 6% ( n =13). The recoveries for the addition of 0.2~0.4 μg/L phosphorous were 95%~110%. The method has been applied to the determination of phosphorous in water samples and soil samples with satisfactory reuslts.
【CateGory Index】: O657.3
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