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《Chinese Journal of Analytieal Chemistry》 2001-02
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Characteristics of Molecular Recognition of Norfloxacin Template Polymer

Guo Hongsheng,He Xiwen,Zhou Jie (Department of Chemistry,Nankai Universtity ,Tianjin 300071) Liang Hong (College of Chemistry and Life Science,Guangxi Normal University,Guilin 541004)  
A molecular template synthetic polymer highly selective for norfloxacinum was prepared by a molecular imprinting technique.The selective binding characteristics of the template polymer was evaluated by Scatchard analysis. The multiple-sites binding model was used to calculate the maximum of binding sites and the dissociation constant. The results showed that the template polymer using methacrylic acid (MAA) as function monomer could form two kinds of binding sites. The dissociation constants were estimated to be Kd1=2.9×10-5 mol/L and Kd2=3.2×10-3mol/L.The selective binding experiment for substiates indicated that the polymer gave much higher affinity and selectivity for norfloxacin than for acid pipemidic and cefalexin.It is possible to be a good adsorption and binding material in the selective enrichment and determination of trace norfloxacin in complex biosamples.
【Fund】: 国家自然科学基金!(No.29775011);; 天津市自然科学基金!(No.003602211);; 广西壮族自治区教育厅科研
【CateGory Index】: O631
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