Spectrophotometric Determination of Trace Germanium in Chinese Medicine by Hydride Generation Technique
Qiu Peihong , Zhang Huajie, Xie Xiafeng (Department of Chemistry, Wenzhou Medical College, Wenzhou 325003)
The hydride generation technique was used to determination of germanium in the presence of L cysteine. The GeH 4 was absorbed and coloured by a mixed solution of 2 (5 NO 2 2 pyridylazo) 5 diethylaminophenyl(5 NO 2 PADAP), NaClO and (CH 2) 6NH +. The maximum absorption of the colour complex was at 564 nm. Its apparent molar absorption coefficient was 1.84×10 5L·mol -1 ·cm -1 . Beer′s law was obeyed for 0～500 μg/L germanium. The recoveries of spiked samples were in the range of 89%～97%, while the relative standard deviation was less than 6.3%. This method is sensitive, simple and reproducible. The method has been applied to the determination of Ge in the chinese medicine with satisfactory result.
【CateGory Index】： O657.3