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《Chinese Journal of Analytieal Chemistry》 2002-01
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Using Affinity Selection to Screen New Drugs in Combinatorial Libraries

Wu Zengru, Xu Xiaojie (College of Chemistry and Molecular Engineering, Peking University, Beijing 100871)  
The method of combinatorial chemistry can provide a large amount of new compounds to screen new drugs, so it is important to screen mixture while the traditional parallel discrete assays can not meet the demand. Compounds which produce biological activity by binding receptor must have affinity to receptor. Based on this property, the biological active compounds can be seperated from the large amount of other compounds. Affinity selection can find the target compounds quickly by identifying the compounds retained by the receptor. The article reviews the forms of the affinity selection screening, its related techniques and application in screening combinatorial libraries. Affinity selection has two main forms: in the first one, the receptor is immobilized to the solid support while retains the target compounds meanwhile the others would be washed off; in the second one, the target protein is incubated with the mixture in solution, and the complexes have great difference from the small moleculars. Affinity selection is also a suitable method in screening natural product extracts in which the components and their amount remain largely unknown.
【CateGory Index】: TQ460
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【Secondary References】
Chinese Journal Full-text Database 2 Hits
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