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《Chinese Journal of Analytieal Chemistry》 2002-04
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Extraction of Lipids with a Low Toxicity Solvent

Han Ju *, Wei Fuxiang (College of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050018) Yun Zihou (Department of Chemistry, Beijing Normal University, Beijing 100875)  
A method for extracting the lipids from meat products with a low toxicity solvent hexane/isopropanol (HIP) is reported. The important factors affecting yield and purity of the extracts were explored, and the optimum extraction conditions and the suitable purification method for the crude extracts were established. The samples were extracted for 10 min with a solvent mixture of hexane and isopropanol (3∶2, V/V ), followed by a wash of the crude extract with 10mL 10 g/L of aqueous sodium sulfate to remove nonlipid contaminants. The extraction yields obtained by both HIP and chloroform/methanol (CM) method were quite similar. The lipid contents and distribution of fatty acids in 6 kinds of meat products were determined, and the results were good agree with those obtained by CM method, but the proposed method was safer, simple and rapid.
【CateGory Index】: O658
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