Studies on Optimization of Ratio of Column Length to Its Diameter for the Separation of Biopolymers by Liquid Chromatography
Zhang Yangjun, Li Xiang, Geng Xindu (Institute of Modern Separation Science, Northwest University, Xi′an, 710069)
The separation of seven proteins was carried out on the chromatographic pie (pie like columns) with 1.0 cm in thickness and different diameters. It is concluded that the ratios of column length to the diameter have little effect on the separation of biopolymers. Also, the different influences of a 200×7.9 mm I.D. column and a 5×50 mm I.D. chromatographic pie with the same interior volume for both on resolution and the differential pressure between these two types of column pie have been compared with each other, the results indicate that the chromatographic pie can significantly lower the back pressure with little decrease in resolution. The injection amount to a 5×50 mm I.D. chromatographic pie and mass recovery determined show that the chromatographic pie can be used for the separation of biopolymers on a preparative scale.
【CateGory Index】： O658